Novel genetic and molecular properties of meiotic recombination protein PRDM9
<p>Meiotic recombination is a fundamental biological process in sexually reproducing organisms, enabling offspring to inherit novel combinations of mutations, and ensuring even segregation of chromosomes into gametes. Recombination is initiated by programmed Double Strand Breaks (DSBs), the ge...
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| Format: | Thesis |
| Language: | English |
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2015
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| _version_ | 1826315804277211136 |
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| author | Altemose, N |
| author2 | Myers, S |
| author_facet | Myers, S Altemose, N |
| author_sort | Altemose, N |
| collection | OXFORD |
| description | <p>Meiotic recombination is a fundamental biological process in sexually reproducing organisms, enabling offspring to inherit novel combinations of mutations, and ensuring even segregation of chromosomes into gametes. Recombination is initiated by programmed Double Strand Breaks (DSBs), the genomic locations of which are determined in most mammals by PRDM9, a rapidly evolving DNA-binding protein. In crosses between different mouse subspecies, certain <em>Prdm9</em> alleles cause infertility in hybrid males, implying a critical role in fertility and speciation. Upon binding to DNA, PRDM9 deposits a histone modification (H3K4me3) typically found in the promoters of expressed genes, suggesting that binding might alter the expression of nearby genes. Many other questions have remained about how PRDM9 initiates recombination, how it causes speciation, and why it evolves so rapidly. This body of work investigates these questions using complementary experimental and analytical methodologies. By generating a map of human PRDM9 binding sites and applying novel sequence analysis methods, I uncovered new DNA-binding modalities of PRDM9 and identified sequence-independent factors that predict binding and recombination outcomes. I also confirmed that PRDM9 can affect gene expression by binding to promoters, identifying candidate regulatory targets in meiosis. Furthermore, I showed that PRDM9’s DNA-binding domain also mediates strong protein-protein interactions that produce PRDM9 multimers, which may play an important functional role. Finally, by generating high-resolution maps of PRDM9 binding in hybrid mice, I provide evidence for a mechanism to explain PRDM9-mediated speciation as a consequence of the joint evolution of PRDM9 and its binding targets. This work reveals that PRDM9 binding on one chromosome strongly impacts DSB formation and/or repair on the homologue, suggesting a novel role for PRDM9 in promoting efficient homology search and DSB repair, both critical for meiotic progression and fertility. One consequence is that PRDM9 may play a wider role in mammalian speciation.</p> |
| first_indexed | 2024-03-06T19:23:42Z |
| format | Thesis |
| id | oxford-uuid:1afe17c3-5f75-4166-8697-7da1471a5230 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:32:55Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oxford-uuid:1afe17c3-5f75-4166-8697-7da1471a52302024-12-01T16:03:07ZNovel genetic and molecular properties of meiotic recombination protein PRDM9Thesishttp://purl.org/coar/resource_type/c_db06uuid:1afe17c3-5f75-4166-8697-7da1471a5230Molecular biologyEvolution (Biology)GeneticsGenetic recombinationMeiosisEnglishORA Deposit2015Altemose, NMyers, S<p>Meiotic recombination is a fundamental biological process in sexually reproducing organisms, enabling offspring to inherit novel combinations of mutations, and ensuring even segregation of chromosomes into gametes. Recombination is initiated by programmed Double Strand Breaks (DSBs), the genomic locations of which are determined in most mammals by PRDM9, a rapidly evolving DNA-binding protein. In crosses between different mouse subspecies, certain <em>Prdm9</em> alleles cause infertility in hybrid males, implying a critical role in fertility and speciation. Upon binding to DNA, PRDM9 deposits a histone modification (H3K4me3) typically found in the promoters of expressed genes, suggesting that binding might alter the expression of nearby genes. Many other questions have remained about how PRDM9 initiates recombination, how it causes speciation, and why it evolves so rapidly. This body of work investigates these questions using complementary experimental and analytical methodologies. By generating a map of human PRDM9 binding sites and applying novel sequence analysis methods, I uncovered new DNA-binding modalities of PRDM9 and identified sequence-independent factors that predict binding and recombination outcomes. I also confirmed that PRDM9 can affect gene expression by binding to promoters, identifying candidate regulatory targets in meiosis. Furthermore, I showed that PRDM9’s DNA-binding domain also mediates strong protein-protein interactions that produce PRDM9 multimers, which may play an important functional role. Finally, by generating high-resolution maps of PRDM9 binding in hybrid mice, I provide evidence for a mechanism to explain PRDM9-mediated speciation as a consequence of the joint evolution of PRDM9 and its binding targets. This work reveals that PRDM9 binding on one chromosome strongly impacts DSB formation and/or repair on the homologue, suggesting a novel role for PRDM9 in promoting efficient homology search and DSB repair, both critical for meiotic progression and fertility. One consequence is that PRDM9 may play a wider role in mammalian speciation.</p> |
| spellingShingle | Molecular biology Evolution (Biology) Genetics Genetic recombination Meiosis Altemose, N Novel genetic and molecular properties of meiotic recombination protein PRDM9 |
| title | Novel genetic and molecular properties of meiotic recombination protein PRDM9 |
| title_full | Novel genetic and molecular properties of meiotic recombination protein PRDM9 |
| title_fullStr | Novel genetic and molecular properties of meiotic recombination protein PRDM9 |
| title_full_unstemmed | Novel genetic and molecular properties of meiotic recombination protein PRDM9 |
| title_short | Novel genetic and molecular properties of meiotic recombination protein PRDM9 |
| title_sort | novel genetic and molecular properties of meiotic recombination protein prdm9 |
| topic | Molecular biology Evolution (Biology) Genetics Genetic recombination Meiosis |
| work_keys_str_mv | AT altemosen novelgeneticandmolecularpropertiesofmeioticrecombinationproteinprdm9 |