Paf1 has distinct roles in transcription elongation and differential transcript fate
RNA polymerase II (Pol2) movement through chromatin and the co-transcriptional processing and fate of nascent transcripts is coordinated by transcription elongation factors (TEFs) such as the polymerase-associated factor 1 (Paf1) but it is not known whether TEFs have gene-specific functions. Using s...
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| Format: | Journal article |
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Cell Press
2017
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| _version_ | 1826261811528204288 |
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| author | Fischl, H Howe, F Furger, A Mellor, J |
| author_facet | Fischl, H Howe, F Furger, A Mellor, J |
| author_sort | Fischl, H |
| collection | OXFORD |
| description | RNA polymerase II (Pol2) movement through chromatin and the co-transcriptional processing and fate of nascent transcripts is coordinated by transcription elongation factors (TEFs) such as the polymerase-associated factor 1 (Paf1) but it is not known whether TEFs have gene-specific functions. Using strand-specific nucleotide resolution techniques, we show that levels of Paf1 on Pol2 vary between genes, are controlled dynamically by environmental factors via promoters, and reflect levels of processing and export factors on the encoded transcript. High levels of Paf1 on Pol2 promote transcript nuclear export while low levels reflect nuclear retention. Strains lacking Paf1 show marked elongation defects, although low levels of Paf1 on Pol2 are sufficient for transcription elongation. Our findings support distinct Paf1 functions: a core general function in transcription elongation, satisfied by the lowest Paf1 levels, and a regulatory function in determining differential transcript fate by varying the level of Paf1 on Pol2. |
| first_indexed | 2024-03-06T19:26:27Z |
| format | Journal article |
| id | oxford-uuid:1be05c2a-5527-49ec-9949-e82eb60c5e91 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T19:26:27Z |
| publishDate | 2017 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:1be05c2a-5527-49ec-9949-e82eb60c5e912022-03-26T11:02:47ZPaf1 has distinct roles in transcription elongation and differential transcript fateJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1be05c2a-5527-49ec-9949-e82eb60c5e91Symplectic Elements at OxfordCell Press2017Fischl, HHowe, FFurger, AMellor, JRNA polymerase II (Pol2) movement through chromatin and the co-transcriptional processing and fate of nascent transcripts is coordinated by transcription elongation factors (TEFs) such as the polymerase-associated factor 1 (Paf1) but it is not known whether TEFs have gene-specific functions. Using strand-specific nucleotide resolution techniques, we show that levels of Paf1 on Pol2 vary between genes, are controlled dynamically by environmental factors via promoters, and reflect levels of processing and export factors on the encoded transcript. High levels of Paf1 on Pol2 promote transcript nuclear export while low levels reflect nuclear retention. Strains lacking Paf1 show marked elongation defects, although low levels of Paf1 on Pol2 are sufficient for transcription elongation. Our findings support distinct Paf1 functions: a core general function in transcription elongation, satisfied by the lowest Paf1 levels, and a regulatory function in determining differential transcript fate by varying the level of Paf1 on Pol2. |
| spellingShingle | Fischl, H Howe, F Furger, A Mellor, J Paf1 has distinct roles in transcription elongation and differential transcript fate |
| title | Paf1 has distinct roles in transcription elongation and differential transcript fate |
| title_full | Paf1 has distinct roles in transcription elongation and differential transcript fate |
| title_fullStr | Paf1 has distinct roles in transcription elongation and differential transcript fate |
| title_full_unstemmed | Paf1 has distinct roles in transcription elongation and differential transcript fate |
| title_short | Paf1 has distinct roles in transcription elongation and differential transcript fate |
| title_sort | paf1 has distinct roles in transcription elongation and differential transcript fate |
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