Polarity-sensitive probes for superresolution stimulated emission depletion microscopy
The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, e...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Cell Press
2017
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| _version_ | 1797056741603540992 |
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| author | Sezgin, E Schneider, F Zilles, V Urbančič, I Garcia, E Waithe, D Klymchenko, A Eggeling, C |
| author_facet | Sezgin, E Schneider, F Zilles, V Urbančič, I Garcia, E Waithe, D Klymchenko, A Eggeling, C |
| author_sort | Sezgin, E |
| collection | OXFORD |
| description | The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles, show the high potential of these dyes for probing nanoscale membrane heterogeneity. |
| first_indexed | 2024-03-06T19:26:54Z |
| format | Journal article |
| id | oxford-uuid:1c0fb0f1-04bd-4a67-bc8e-563aaba4c495 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:26:54Z |
| publishDate | 2017 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:1c0fb0f1-04bd-4a67-bc8e-563aaba4c4952022-03-26T11:03:39ZPolarity-sensitive probes for superresolution stimulated emission depletion microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1c0fb0f1-04bd-4a67-bc8e-563aaba4c495EnglishSymplectic Elements at OxfordCell Press2017Sezgin, ESchneider, FZilles, VUrbančič, IGarcia, EWaithe, DKlymchenko, AEggeling, CThe lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles, show the high potential of these dyes for probing nanoscale membrane heterogeneity. |
| spellingShingle | Sezgin, E Schneider, F Zilles, V Urbančič, I Garcia, E Waithe, D Klymchenko, A Eggeling, C Polarity-sensitive probes for superresolution stimulated emission depletion microscopy |
| title | Polarity-sensitive probes for superresolution stimulated emission depletion microscopy |
| title_full | Polarity-sensitive probes for superresolution stimulated emission depletion microscopy |
| title_fullStr | Polarity-sensitive probes for superresolution stimulated emission depletion microscopy |
| title_full_unstemmed | Polarity-sensitive probes for superresolution stimulated emission depletion microscopy |
| title_short | Polarity-sensitive probes for superresolution stimulated emission depletion microscopy |
| title_sort | polarity sensitive probes for superresolution stimulated emission depletion microscopy |
| work_keys_str_mv | AT sezgine polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy AT schneiderf polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy AT zillesv polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy AT urbancici polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy AT garciae polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy AT waithed polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy AT klymchenkoa polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy AT eggelingc polaritysensitiveprobesforsuperresolutionstimulatedemissiondepletionmicroscopy |