Monocyte activation and cytokine production in Malawian children presenting with P. falciparum malaria
Malaria in malaria-naïve adults is associated with an inflammatory response characterised by expression of specific activation markers on innate immune cells. Here we investigate activation and adhesion marker expression, and cytokine production in monocytes from children presenting with cerebral ma...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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Wiley
2016
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| _version_ | 1797056794269319168 |
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| author | Mandala, W Msefula, C Gondwe, E Drayson, M Molyneux, M MacLennan, C |
| author_facet | Mandala, W Msefula, C Gondwe, E Drayson, M Molyneux, M MacLennan, C |
| author_sort | Mandala, W |
| collection | OXFORD |
| description | Malaria in malaria-naïve adults is associated with an inflammatory response characterised by expression of specific activation markers on innate immune cells. Here we investigate activation and adhesion marker expression, and cytokine production in monocytes from children presenting with cerebral malaria (CM, n=36), severe malarial anaemia (SMA, n=42) or uncomplicated malaria (UM, n= 66), and healthy aparasitemic children (n=52) in Blantyre, Malawi. In all malaria groups, but particularly in the two severe malaria groups, monocyte expression of CD11b, CD11c, CD18, HLA-DR and CD86, and percentages of TNFα and IL-6-producing monocytes were lower than in healthy controls, while expression of CD11a, TLR2 and TLR4 was lower in children with severe malaria compared with controls. These levels mostly normalised during convalescence, but percentages of cytokine-producing monocytes remained suppressed in children with SMA. In all malaria groups, especially the SMA group, a greater proportion of monocytes were loaded with haemozoin than among controls. In a P. falciparum hyperendemic area, monocytes in children with acute symptomatic malaria have reduced expression of adhesion molecules and activation markers and reduced inflammatory cytokine production. This immune suppression could be due to accumulation of haemozoin and/or previous exposure to P. falciparum. This article is protected by copyright. All rights reserved. |
| first_indexed | 2024-03-06T19:27:31Z |
| format | Journal article |
| id | oxford-uuid:1c44be9c-4a46-42fc-b7f4-b2db8f302e7f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:27:31Z |
| publishDate | 2016 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:1c44be9c-4a46-42fc-b7f4-b2db8f302e7f2022-03-26T11:04:45ZMonocyte activation and cytokine production in Malawian children presenting with P. falciparum malariaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1c44be9c-4a46-42fc-b7f4-b2db8f302e7fEnglishSymplectic Elements at OxfordWiley2016Mandala, WMsefula, CGondwe, EDrayson, MMolyneux, MMacLennan, CMalaria in malaria-naïve adults is associated with an inflammatory response characterised by expression of specific activation markers on innate immune cells. Here we investigate activation and adhesion marker expression, and cytokine production in monocytes from children presenting with cerebral malaria (CM, n=36), severe malarial anaemia (SMA, n=42) or uncomplicated malaria (UM, n= 66), and healthy aparasitemic children (n=52) in Blantyre, Malawi. In all malaria groups, but particularly in the two severe malaria groups, monocyte expression of CD11b, CD11c, CD18, HLA-DR and CD86, and percentages of TNFα and IL-6-producing monocytes were lower than in healthy controls, while expression of CD11a, TLR2 and TLR4 was lower in children with severe malaria compared with controls. These levels mostly normalised during convalescence, but percentages of cytokine-producing monocytes remained suppressed in children with SMA. In all malaria groups, especially the SMA group, a greater proportion of monocytes were loaded with haemozoin than among controls. In a P. falciparum hyperendemic area, monocytes in children with acute symptomatic malaria have reduced expression of adhesion molecules and activation markers and reduced inflammatory cytokine production. This immune suppression could be due to accumulation of haemozoin and/or previous exposure to P. falciparum. This article is protected by copyright. All rights reserved. |
| spellingShingle | Mandala, W Msefula, C Gondwe, E Drayson, M Molyneux, M MacLennan, C Monocyte activation and cytokine production in Malawian children presenting with P. falciparum malaria |
| title | Monocyte activation and cytokine production in Malawian children presenting with P. falciparum malaria |
| title_full | Monocyte activation and cytokine production in Malawian children presenting with P. falciparum malaria |
| title_fullStr | Monocyte activation and cytokine production in Malawian children presenting with P. falciparum malaria |
| title_full_unstemmed | Monocyte activation and cytokine production in Malawian children presenting with P. falciparum malaria |
| title_short | Monocyte activation and cytokine production in Malawian children presenting with P. falciparum malaria |
| title_sort | monocyte activation and cytokine production in malawian children presenting with p falciparum malaria |
| work_keys_str_mv | AT mandalaw monocyteactivationandcytokineproductioninmalawianchildrenpresentingwithpfalciparummalaria AT msefulac monocyteactivationandcytokineproductioninmalawianchildrenpresentingwithpfalciparummalaria AT gondwee monocyteactivationandcytokineproductioninmalawianchildrenpresentingwithpfalciparummalaria AT draysonm monocyteactivationandcytokineproductioninmalawianchildrenpresentingwithpfalciparummalaria AT molyneuxm monocyteactivationandcytokineproductioninmalawianchildrenpresentingwithpfalciparummalaria AT maclennanc monocyteactivationandcytokineproductioninmalawianchildrenpresentingwithpfalciparummalaria |