Using molecular rotors to probe gelation.
A series of fluorescent probes, including a number of molecular rotors, have been used to follow the self-assembly of dipeptide-based low molecular weight gelators. We show that these probes can be used to gain an insight into the assembly process. Thioflavin T, a commonly used stain for β-sheets, a...
| Автори: | , , , , , , |
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| Формат: | Journal article |
| Мова: | English |
| Опубліковано: |
Royal Society of Chemistry
2015
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| _version_ | 1826262045734993920 |
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| author | Raeburn, J Chen, L Awhida, S Deller, R Vatish, M Gibson, M Adams, D |
| author_facet | Raeburn, J Chen, L Awhida, S Deller, R Vatish, M Gibson, M Adams, D |
| author_sort | Raeburn, J |
| collection | OXFORD |
| description | A series of fluorescent probes, including a number of molecular rotors, have been used to follow the self-assembly of dipeptide-based low molecular weight gelators. We show that these probes can be used to gain an insight into the assembly process. Thioflavin T, a commonly used stain for β-sheets, appears to act as a molecular rotor in these gelling systems, with the fluorescence data closely matching that of other rotors. The molecular rotor was incorporated into an assay system with glucose oxidase to enable glucose-concentration specific gelation and hence generating a fluorescent output. Applying this system to urine from patients with various levels of glycosuria (a symptom of diabetes), it was found to provide excellent correlation with different clinical assessments of diabetes. This demonstrates a new concept in gelation-linked biosensing for a real clinical problem. |
| first_indexed | 2024-03-06T19:30:09Z |
| format | Journal article |
| id | oxford-uuid:1d2d8894-d6ea-4c97-afcf-48d21795ed77 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:30:09Z |
| publishDate | 2015 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:1d2d8894-d6ea-4c97-afcf-48d21795ed772022-03-26T11:09:28ZUsing molecular rotors to probe gelation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1d2d8894-d6ea-4c97-afcf-48d21795ed77EnglishSymplectic Elements at OxfordRoyal Society of Chemistry2015Raeburn, JChen, LAwhida, SDeller, RVatish, MGibson, MAdams, DA series of fluorescent probes, including a number of molecular rotors, have been used to follow the self-assembly of dipeptide-based low molecular weight gelators. We show that these probes can be used to gain an insight into the assembly process. Thioflavin T, a commonly used stain for β-sheets, appears to act as a molecular rotor in these gelling systems, with the fluorescence data closely matching that of other rotors. The molecular rotor was incorporated into an assay system with glucose oxidase to enable glucose-concentration specific gelation and hence generating a fluorescent output. Applying this system to urine from patients with various levels of glycosuria (a symptom of diabetes), it was found to provide excellent correlation with different clinical assessments of diabetes. This demonstrates a new concept in gelation-linked biosensing for a real clinical problem. |
| spellingShingle | Raeburn, J Chen, L Awhida, S Deller, R Vatish, M Gibson, M Adams, D Using molecular rotors to probe gelation. |
| title | Using molecular rotors to probe gelation. |
| title_full | Using molecular rotors to probe gelation. |
| title_fullStr | Using molecular rotors to probe gelation. |
| title_full_unstemmed | Using molecular rotors to probe gelation. |
| title_short | Using molecular rotors to probe gelation. |
| title_sort | using molecular rotors to probe gelation |
| work_keys_str_mv | AT raeburnj usingmolecularrotorstoprobegelation AT chenl usingmolecularrotorstoprobegelation AT awhidas usingmolecularrotorstoprobegelation AT dellerr usingmolecularrotorstoprobegelation AT vatishm usingmolecularrotorstoprobegelation AT gibsonm usingmolecularrotorstoprobegelation AT adamsd usingmolecularrotorstoprobegelation |