Flow and nutrient transport problems in rotating bioreactor systems
<p>Motivated by applications in tissue engineering, this thesis is concerned with the flow through and around a free-moving porous tissue construct (TC) within a high-aspect-ratio vessel (HARV) bioreactor. We formalise and extend various results for flow within a Hele-Shaw cell containing a po...
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| Format: | Thesis |
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2014
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| author | Dalwadi, M Chapman, S Oliver, J Waters, S |
| author2 | Chapman, S |
| author_facet | Chapman, S Dalwadi, M Chapman, S Oliver, J Waters, S |
| author_sort | Dalwadi, M |
| collection | OXFORD |
| description | <p>Motivated by applications in tissue engineering, this thesis is concerned with the flow through and around a free-moving porous tissue construct (TC) within a high-aspect-ratio vessel (HARV) bioreactor. We formalise and extend various results for flow within a Hele-Shaw cell containing a porous obstacle. We also consider the impact of the flow on related nutrient transport problems.</p> <p>The HARV bioreactor is a cylinder with circular cross-section which rotates about its axis at a constant rate, and is filled with a nutrient-rich culture medium. The porous TC is modelled as a rigid porous cylinder with circular cross-section and is fully saturated with the fluid.</p> <p>We formulate the flow problem for a porous TC (governed by Darcy's equations) within a HARV bioreactor (governed by the Navier-Stokes equations). We couple the two regions via appropriate interfacial conditions which are derived by consideration of the intricate boundary-layer structure close to the TC surface. By exploiting various small parameters, we simplify the system of equations by performing an asymptotic analysis, and investigate the resulting system for the flow due to a prescribed TC motion.</p> <p>The motion of the TC is determined by analysis of the force and torque acting upon it, and the resulting equations of motion (which are coupled to the flow) are investigated. The short-time TC behaviour is periodic, but we are able to study the long-time drift from this periodic solution by considering the effect of inertia using a multiple-scale analysis. We find that, contrary to received wisdom, inertia affects TC drift on a similar timescale to tissue growth.</p> <p>Finally, we consider the advection of nutrient through the bioreactor and TC, and investigate the problem of nutrient advection-diffusion for a simplified model involving nutrient uptake.</p> |
| first_indexed | 2024-03-06T19:30:55Z |
| format | Thesis |
| id | oxford-uuid:1d7298b7-cdf5-4240-a79c-b7b69f662c1a |
| institution | University of Oxford |
| last_indexed | 2024-12-09T03:33:26Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:1d7298b7-cdf5-4240-a79c-b7b69f662c1a2024-12-01T16:37:23ZFlow and nutrient transport problems in rotating bioreactor systemsThesishttp://purl.org/coar/resource_type/c_db06uuid:1d7298b7-cdf5-4240-a79c-b7b69f662c1aFluid mechanics (mathematics)MathematicsPartial differential equationsMathematical biologyORA Deposit2014Dalwadi, MChapman, SOliver, JWaters, SChapman, SOliver, JWaters, S<p>Motivated by applications in tissue engineering, this thesis is concerned with the flow through and around a free-moving porous tissue construct (TC) within a high-aspect-ratio vessel (HARV) bioreactor. We formalise and extend various results for flow within a Hele-Shaw cell containing a porous obstacle. We also consider the impact of the flow on related nutrient transport problems.</p> <p>The HARV bioreactor is a cylinder with circular cross-section which rotates about its axis at a constant rate, and is filled with a nutrient-rich culture medium. The porous TC is modelled as a rigid porous cylinder with circular cross-section and is fully saturated with the fluid.</p> <p>We formulate the flow problem for a porous TC (governed by Darcy's equations) within a HARV bioreactor (governed by the Navier-Stokes equations). We couple the two regions via appropriate interfacial conditions which are derived by consideration of the intricate boundary-layer structure close to the TC surface. By exploiting various small parameters, we simplify the system of equations by performing an asymptotic analysis, and investigate the resulting system for the flow due to a prescribed TC motion.</p> <p>The motion of the TC is determined by analysis of the force and torque acting upon it, and the resulting equations of motion (which are coupled to the flow) are investigated. The short-time TC behaviour is periodic, but we are able to study the long-time drift from this periodic solution by considering the effect of inertia using a multiple-scale analysis. We find that, contrary to received wisdom, inertia affects TC drift on a similar timescale to tissue growth.</p> <p>Finally, we consider the advection of nutrient through the bioreactor and TC, and investigate the problem of nutrient advection-diffusion for a simplified model involving nutrient uptake.</p> |
| spellingShingle | Fluid mechanics (mathematics) Mathematics Partial differential equations Mathematical biology Dalwadi, M Chapman, S Oliver, J Waters, S Flow and nutrient transport problems in rotating bioreactor systems |
| title | Flow and nutrient transport problems in rotating bioreactor systems |
| title_full | Flow and nutrient transport problems in rotating bioreactor systems |
| title_fullStr | Flow and nutrient transport problems in rotating bioreactor systems |
| title_full_unstemmed | Flow and nutrient transport problems in rotating bioreactor systems |
| title_short | Flow and nutrient transport problems in rotating bioreactor systems |
| title_sort | flow and nutrient transport problems in rotating bioreactor systems |
| topic | Fluid mechanics (mathematics) Mathematics Partial differential equations Mathematical biology |
| work_keys_str_mv | AT dalwadim flowandnutrienttransportproblemsinrotatingbioreactorsystems AT chapmans flowandnutrienttransportproblemsinrotatingbioreactorsystems AT oliverj flowandnutrienttransportproblemsinrotatingbioreactorsystems AT waterss flowandnutrienttransportproblemsinrotatingbioreactorsystems |