1H and 31P NMR investigation of gadolinium uptake in maize roots
Low-resolution 1H NMR and high-resolution 31P NMR were used to study the absorption of gadolinium (Gd) by the root tissues of maize seedlings. Gd was supplied as either Gd3+ or Gd(DTPA)2-, and its presence in the tissue caused changes in the relaxation properties of the tissue water and of various p...
| Egile Nagusiak: | , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
1990
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| _version_ | 1826262119434158080 |
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| author | Quiquampoix, H Ratcliffe, R Ratković, S Ratković, S Vučinić, Ž |
| author_facet | Quiquampoix, H Ratcliffe, R Ratković, S Ratković, S Vučinić, Ž |
| author_sort | Quiquampoix, H |
| collection | OXFORD |
| description | Low-resolution 1H NMR and high-resolution 31P NMR were used to study the absorption of gadolinium (Gd) by the root tissues of maize seedlings. Gd was supplied as either Gd3+ or Gd(DTPA)2-, and its presence in the tissue caused changes in the relaxation properties of the tissue water and of various phosphorylated metabolites. 1H NMR relaxation measurements indicated that soluble Gd accumulated in the tissue more rapidly with Gd(DTPA)2- than with Gd3+, and this was attributed to the precipitation of Gd3+ in the root. Electron microscopy and x-ray microanalysis confirmed the presence of deposits containing Gd and phosphorus (P) in the extracellular space of the tissue. 31P NMR showed the presence of soluble Gd in both the cytoplasm and the vacuole of roots treated with Gd3+ and, thus, proved that Gd3+ can reach the symplast, contrary to the usual assumption about the lanthanides. Evidence for the immobilization of P by Gd3+ and for the vacuolar breakdown of Gd(DTPA)2- was also obtained from the 31P NMR spectra. © 1990. |
| first_indexed | 2024-03-06T19:31:21Z |
| format | Journal article |
| id | oxford-uuid:1d8e0d9b-c0c1-4d04-bc5c-3638d94371cf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:31:21Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:1d8e0d9b-c0c1-4d04-bc5c-3638d94371cf2022-03-26T11:11:37Z 1H and 31P NMR investigation of gadolinium uptake in maize rootsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1d8e0d9b-c0c1-4d04-bc5c-3638d94371cfEnglishSymplectic Elements at Oxford1990Quiquampoix, HRatcliffe, RRatković, SRatković, SVučinić, ŽLow-resolution 1H NMR and high-resolution 31P NMR were used to study the absorption of gadolinium (Gd) by the root tissues of maize seedlings. Gd was supplied as either Gd3+ or Gd(DTPA)2-, and its presence in the tissue caused changes in the relaxation properties of the tissue water and of various phosphorylated metabolites. 1H NMR relaxation measurements indicated that soluble Gd accumulated in the tissue more rapidly with Gd(DTPA)2- than with Gd3+, and this was attributed to the precipitation of Gd3+ in the root. Electron microscopy and x-ray microanalysis confirmed the presence of deposits containing Gd and phosphorus (P) in the extracellular space of the tissue. 31P NMR showed the presence of soluble Gd in both the cytoplasm and the vacuole of roots treated with Gd3+ and, thus, proved that Gd3+ can reach the symplast, contrary to the usual assumption about the lanthanides. Evidence for the immobilization of P by Gd3+ and for the vacuolar breakdown of Gd(DTPA)2- was also obtained from the 31P NMR spectra. © 1990. |
| spellingShingle | Quiquampoix, H Ratcliffe, R Ratković, S Ratković, S Vučinić, Ž 1H and 31P NMR investigation of gadolinium uptake in maize roots |
| title |
1H and 31P NMR investigation of gadolinium uptake in maize roots |
| title_full |
1H and 31P NMR investigation of gadolinium uptake in maize roots |
| title_fullStr |
1H and 31P NMR investigation of gadolinium uptake in maize roots |
| title_full_unstemmed |
1H and 31P NMR investigation of gadolinium uptake in maize roots |
| title_short |
1H and 31P NMR investigation of gadolinium uptake in maize roots |
| title_sort | 1h and 31p nmr investigation of gadolinium uptake in maize roots |
| work_keys_str_mv | AT quiquampoixh 1hand31pnmrinvestigationofgadoliniumuptakeinmaizeroots AT ratcliffer 1hand31pnmrinvestigationofgadoliniumuptakeinmaizeroots AT ratkovics 1hand31pnmrinvestigationofgadoliniumuptakeinmaizeroots AT ratkovics 1hand31pnmrinvestigationofgadoliniumuptakeinmaizeroots AT vucinicz 1hand31pnmrinvestigationofgadoliniumuptakeinmaizeroots |