FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells

FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells

A major challenge in single-molecule imaging is tracking the dynamics of proteins or complexes for long periods of time in the dense environments found in living cells. Here, we introduce the concept of using FRET to enhance the photophysical properties of photo-modulatable (PM) fluorophores commonl...

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Бібліографічні деталі
Автори: Basu, S, Needham, L, Lando, D, Taylor, E, Wohlfahrt, K, Shah, D, Boucher, W, Tan, Y, Bates, L, Tkachenko, O, Cramard, J, Lagerholm, B, Eggeling, C, Hendrich, B, Klenerman, D, Lee, S, Laue, E
Формат: Journal article
Мова:English
Опубліковано: Nature Publishing Group 2018

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