Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging.
Cardiac architecture is fundamental to cardiac function and can be assessed non-invasively with diffusion tensor imaging (DTI). Here, we aimed to overcome technical challenges in ex vivoDTI in order to extract fine anatomical details and to provide novel insights in the 3D structure of the heart. An...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Nature Publishing Group
2016
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| _version_ | 1797057192923234304 |
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| author | Teh, I McClymont, D Burton, R Maguire, M Whittington, H Lygate, C Kohl, P Schneider, J |
| author_facet | Teh, I McClymont, D Burton, R Maguire, M Whittington, H Lygate, C Kohl, P Schneider, J |
| author_sort | Teh, I |
| collection | OXFORD |
| description | Cardiac architecture is fundamental to cardiac function and can be assessed non-invasively with diffusion tensor imaging (DTI). Here, we aimed to overcome technical challenges in ex vivoDTI in order to extract fine anatomical details and to provide novel insights in the 3D structure of the heart. An integrated set of methods was implemented in ex vivorat hearts, including dynamic receiver gain adjustment, gradient system scaling calibration, prospective adjustment of diffusion gradients, and interleaving of diffusion-weighted and non-diffusion-weighted scans. Together, these methods enhanced SNR and spatial resolution, minimised orientation bias in diffusion-weighting, and reduced temperature variation, enabling detection of tissue structures such as cell alignment in atria, valves and vessels at an unprecedented level of detail. Improved confidence in eigenvector reproducibility enabled tracking of myolaminar structures as a basis for segmentation of functional groups of cardiomyocytes. Ex vivoDTI facilitates acquisition of high quality structural data that complements readily available in vivocardiac functional and anatomical MRI. The improvements presented here will facilitate next generation virtual models integrating micro-structural and electro-mechanical properties of the heart. |
| first_indexed | 2024-03-06T19:32:46Z |
| format | Journal article |
| id | oxford-uuid:1e0a3057-7a97-41f0-b0ee-7d9d56912a72 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:32:46Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:1e0a3057-7a97-41f0-b0ee-7d9d56912a722022-03-26T11:14:11ZResolving fine cardiac structures in rats with high-resolution diffusion tensor imaging.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1e0a3057-7a97-41f0-b0ee-7d9d56912a72EnglishSymplectic Elements at OxfordNature Publishing Group2016Teh, IMcClymont, DBurton, RMaguire, MWhittington, HLygate, CKohl, PSchneider, JCardiac architecture is fundamental to cardiac function and can be assessed non-invasively with diffusion tensor imaging (DTI). Here, we aimed to overcome technical challenges in ex vivoDTI in order to extract fine anatomical details and to provide novel insights in the 3D structure of the heart. An integrated set of methods was implemented in ex vivorat hearts, including dynamic receiver gain adjustment, gradient system scaling calibration, prospective adjustment of diffusion gradients, and interleaving of diffusion-weighted and non-diffusion-weighted scans. Together, these methods enhanced SNR and spatial resolution, minimised orientation bias in diffusion-weighting, and reduced temperature variation, enabling detection of tissue structures such as cell alignment in atria, valves and vessels at an unprecedented level of detail. Improved confidence in eigenvector reproducibility enabled tracking of myolaminar structures as a basis for segmentation of functional groups of cardiomyocytes. Ex vivoDTI facilitates acquisition of high quality structural data that complements readily available in vivocardiac functional and anatomical MRI. The improvements presented here will facilitate next generation virtual models integrating micro-structural and electro-mechanical properties of the heart. |
| spellingShingle | Teh, I McClymont, D Burton, R Maguire, M Whittington, H Lygate, C Kohl, P Schneider, J Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging. |
| title | Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging. |
| title_full | Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging. |
| title_fullStr | Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging. |
| title_full_unstemmed | Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging. |
| title_short | Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging. |
| title_sort | resolving fine cardiac structures in rats with high resolution diffusion tensor imaging |
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