The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation.
Centrosomes are important cell organizers. They consist of a pair of centrioles surrounded by pericentriolar material (PCM) that expands dramatically during mitosis-a process termed centrosome maturation. How centrosomes mature remains mysterious. Here, we identify a domain in Drosophila Cnn that ap...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Cell Press
2014
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| _version_ | 1826262218729062400 |
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| author | Conduit, P Feng, Z Richens, J Baumbach, J Wainman, A Bakshi, S Dobbelaere, J Johnson, S Lea, S Raff, J |
| author_facet | Conduit, P Feng, Z Richens, J Baumbach, J Wainman, A Bakshi, S Dobbelaere, J Johnson, S Lea, S Raff, J |
| author_sort | Conduit, P |
| collection | OXFORD |
| description | Centrosomes are important cell organizers. They consist of a pair of centrioles surrounded by pericentriolar material (PCM) that expands dramatically during mitosis-a process termed centrosome maturation. How centrosomes mature remains mysterious. Here, we identify a domain in Drosophila Cnn that appears to be phosphorylated by Polo/Plk1 specifically at centrosomes during mitosis. The phosphorylation promotes the assembly of a Cnn scaffold around the centrioles that is in constant flux, with Cnn molecules recruited continuously around the centrioles as the scaffold spreads slowly outward. Mutations that block Cnn phosphorylation strongly inhibit scaffold assembly and centrosome maturation, whereas phosphomimicking mutations allow Cnn to multimerize in vitro and to spontaneously form cytoplasmic scaffolds in vivo that organize microtubules independently of centrosomes. We conclude that Polo/Plk1 initiates the phosphorylation-dependent assembly of a Cnn scaffold around centrioles that is essential for efficient centrosome maturation in flies. |
| first_indexed | 2024-03-06T19:32:55Z |
| format | Journal article |
| id | oxford-uuid:1e15ffbd-6422-4c29-a09c-bb59740d2388 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:32:55Z |
| publishDate | 2014 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:1e15ffbd-6422-4c29-a09c-bb59740d23882022-03-26T11:14:26ZThe centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1e15ffbd-6422-4c29-a09c-bb59740d2388EnglishSymplectic Elements at OxfordCell Press2014Conduit, PFeng, ZRichens, JBaumbach, JWainman, ABakshi, SDobbelaere, JJohnson, SLea, SRaff, JCentrosomes are important cell organizers. They consist of a pair of centrioles surrounded by pericentriolar material (PCM) that expands dramatically during mitosis-a process termed centrosome maturation. How centrosomes mature remains mysterious. Here, we identify a domain in Drosophila Cnn that appears to be phosphorylated by Polo/Plk1 specifically at centrosomes during mitosis. The phosphorylation promotes the assembly of a Cnn scaffold around the centrioles that is in constant flux, with Cnn molecules recruited continuously around the centrioles as the scaffold spreads slowly outward. Mutations that block Cnn phosphorylation strongly inhibit scaffold assembly and centrosome maturation, whereas phosphomimicking mutations allow Cnn to multimerize in vitro and to spontaneously form cytoplasmic scaffolds in vivo that organize microtubules independently of centrosomes. We conclude that Polo/Plk1 initiates the phosphorylation-dependent assembly of a Cnn scaffold around centrioles that is essential for efficient centrosome maturation in flies. |
| spellingShingle | Conduit, P Feng, Z Richens, J Baumbach, J Wainman, A Bakshi, S Dobbelaere, J Johnson, S Lea, S Raff, J The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. |
| title | The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. |
| title_full | The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. |
| title_fullStr | The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. |
| title_full_unstemmed | The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. |
| title_short | The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. |
| title_sort | centrosome specific phosphorylation of cnn by polo plk1 drives cnn scaffold assembly and centrosome maturation |
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