A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence.
We have analysed the sequences required for cleavage and polyadenylation in the intronless melanocortin 4 receptor (MC4R) pre-mRNA. Unlike other intronless genes, 3'end processing of the MC4R primary transcript is independent of any auxiliary sequence elements and only requires the core poly(A)...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2010
|
| _version_ | 1826262280724021248 |
|---|---|
| author | Nunes, N Li, W Tian, B Furger, A |
| author_facet | Nunes, N Li, W Tian, B Furger, A |
| author_sort | Nunes, N |
| collection | OXFORD |
| description | We have analysed the sequences required for cleavage and polyadenylation in the intronless melanocortin 4 receptor (MC4R) pre-mRNA. Unlike other intronless genes, 3'end processing of the MC4R primary transcript is independent of any auxiliary sequence elements and only requires the core poly(A) sequences. Mutation of the AUUAAA hexamer had little effect on MC4R 3'end processing but small changes in the short DSE severely reduced cleavage efficiency. The MC4R poly(A) site requires only the DSE and an A-rich upstream sequence to direct efficient cleavage and polyadenylation. Our observation may be highly relevant for the understanding of how human noncanonical poly(A) sites are recognised. This is supported by a genome-wide analysis of over 10 000 poly(A) sites where we show that many human noncanonical poly(A) signals contain A-rich upstream sequences and tend to have a higher frequency of U and GU nucleotides in their DSE compared with canonical poly(A) signals. The importance of A-rich elements for noncanonical poly(A) site recognition was confirmed by mutational analysis of the human JUNB gene, which contains an A-rich noncanonical poly(A) signal. |
| first_indexed | 2024-03-06T19:33:54Z |
| format | Journal article |
| id | oxford-uuid:1e6415e4-b3df-42d0-8cd2-56164e2b05f6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:33:54Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:1e6415e4-b3df-42d0-8cd2-56164e2b05f62022-03-26T11:16:03ZA functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1e6415e4-b3df-42d0-8cd2-56164e2b05f6EnglishSymplectic Elements at Oxford2010Nunes, NLi, WTian, BFurger, AWe have analysed the sequences required for cleavage and polyadenylation in the intronless melanocortin 4 receptor (MC4R) pre-mRNA. Unlike other intronless genes, 3'end processing of the MC4R primary transcript is independent of any auxiliary sequence elements and only requires the core poly(A) sequences. Mutation of the AUUAAA hexamer had little effect on MC4R 3'end processing but small changes in the short DSE severely reduced cleavage efficiency. The MC4R poly(A) site requires only the DSE and an A-rich upstream sequence to direct efficient cleavage and polyadenylation. Our observation may be highly relevant for the understanding of how human noncanonical poly(A) sites are recognised. This is supported by a genome-wide analysis of over 10 000 poly(A) sites where we show that many human noncanonical poly(A) signals contain A-rich upstream sequences and tend to have a higher frequency of U and GU nucleotides in their DSE compared with canonical poly(A) signals. The importance of A-rich elements for noncanonical poly(A) site recognition was confirmed by mutational analysis of the human JUNB gene, which contains an A-rich noncanonical poly(A) signal. |
| spellingShingle | Nunes, N Li, W Tian, B Furger, A A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence. |
| title | A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence. |
| title_full | A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence. |
| title_fullStr | A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence. |
| title_full_unstemmed | A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence. |
| title_short | A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence. |
| title_sort | functional human poly a site requires only a potent dse and an a rich upstream sequence |
| work_keys_str_mv | AT nunesn afunctionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence AT liw afunctionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence AT tianb afunctionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence AT furgera afunctionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence AT nunesn functionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence AT liw functionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence AT tianb functionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence AT furgera functionalhumanpolyasiterequiresonlyapotentdseandanarichupstreamsequence |