Studies on the functional heterogeneity of rat T cells using monoclonal antibodies
<p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">A new monoclonal antibody against rat lymphocytes is described. This antibody, designated MRC-OX22, recognises an antigen found on all peripheral B cells, all suppressor/cytotoxic lymphocytes, but only on some helper cells, a...
| Main Authors: | , |
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| Format: | Thesis |
| Language: | English |
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1983
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| _version_ | 1817932174399111168 |
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| author | Spickett, G Spickett, G.P. |
| author2 | Mason, D |
| author_facet | Mason, D Spickett, G Spickett, G.P. |
| author_sort | Spickett, G |
| collection | OXFORD |
| description | <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">A new monoclonal antibody against rat lymphocytes is described. This antibody, designated MRC-OX22, recognises an antigen found on all peripheral B cells, all suppressor/cytotoxic lymphocytes, but only on some helper cells, as defined by the W3/25 antibody. The antigen is found on a significant number of bone marrow cells, but only on 3% of thymocytes, which were located by immunoperoxidase staining at the cortico-medullary junction.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">The phenotypic division of the W3/25<sup>+</sup> peripheral T cells, is mirrored functionally. By using the popliteal lymph node assay, and the induction of lethal graft-versus-host disease as indicators of alloreactivity, it is found that the alloreactive T cells have the phenotype MRC-OX22<sup>+</sup> ,W3/25<sup>+</sup>. In contrast, using an adoptive transfer system to assess T cell help for B cell antibody production, the phenotype of the T<sub>helper</sub> for B cells is shown to have the phenotype MRC-OX22<sup>-</sup>, W3/25<sup>+</sup>. Evidence is also presented that the phenotype of the T<sub>helper</sub> cell for B cells is stable after priming and boosting.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">The phenotype of the memory B cell is shown to be MRC-OX22<sup>+</sup>, and the cell responsible for allogeneic suppression is also shown to be MRC-OX22<sup>+</sup>, using the adoptive transfer system for antibody formation.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">The significance of the functional heterogeneity of the W3/25<sup>+</sup> subset is discussed, and the biochemical nature of the antigen recognised by MRC-OX22 is reviewed, and compared with possible homologues in mouse and man.</p> |
| first_indexed | 2024-03-06T19:34:24Z |
| format | Thesis |
| id | oxford-uuid:1e8c9ecb-af74-4b33-a5c5-71a20e31f5dc |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:33:43Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:1e8c9ecb-af74-4b33-a5c5-71a20e31f5dc2024-12-01T17:17:01ZStudies on the functional heterogeneity of rat T cells using monoclonal antibodiesThesishttp://purl.org/coar/resource_type/c_db06uuid:1e8c9ecb-af74-4b33-a5c5-71a20e31f5dcMonoclonal antibodiesAntigensB cellsLymphocytesT cellsEnglishPolonsky Theses Digitisation Project1983Spickett, GSpickett, G.P.Mason, DMason, D<p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">A new monoclonal antibody against rat lymphocytes is described. This antibody, designated MRC-OX22, recognises an antigen found on all peripheral B cells, all suppressor/cytotoxic lymphocytes, but only on some helper cells, as defined by the W3/25 antibody. The antigen is found on a significant number of bone marrow cells, but only on 3% of thymocytes, which were located by immunoperoxidase staining at the cortico-medullary junction.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">The phenotypic division of the W3/25<sup>+</sup> peripheral T cells, is mirrored functionally. By using the popliteal lymph node assay, and the induction of lethal graft-versus-host disease as indicators of alloreactivity, it is found that the alloreactive T cells have the phenotype MRC-OX22<sup>+</sup> ,W3/25<sup>+</sup>. In contrast, using an adoptive transfer system to assess T cell help for B cell antibody production, the phenotype of the T<sub>helper</sub> for B cells is shown to have the phenotype MRC-OX22<sup>-</sup>, W3/25<sup>+</sup>. Evidence is also presented that the phenotype of the T<sub>helper</sub> cell for B cells is stable after priming and boosting.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">The phenotype of the memory B cell is shown to be MRC-OX22<sup>+</sup>, and the cell responsible for allogeneic suppression is also shown to be MRC-OX22<sup>+</sup>, using the adoptive transfer system for antibody formation.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">The significance of the functional heterogeneity of the W3/25<sup>+</sup> subset is discussed, and the biochemical nature of the antigen recognised by MRC-OX22 is reviewed, and compared with possible homologues in mouse and man.</p> |
| spellingShingle | Monoclonal antibodies Antigens B cells Lymphocytes T cells Spickett, G Spickett, G.P. Studies on the functional heterogeneity of rat T cells using monoclonal antibodies |
| title | Studies on the functional heterogeneity of rat T cells using monoclonal antibodies |
| title_full | Studies on the functional heterogeneity of rat T cells using monoclonal antibodies |
| title_fullStr | Studies on the functional heterogeneity of rat T cells using monoclonal antibodies |
| title_full_unstemmed | Studies on the functional heterogeneity of rat T cells using monoclonal antibodies |
| title_short | Studies on the functional heterogeneity of rat T cells using monoclonal antibodies |
| title_sort | studies on the functional heterogeneity of rat t cells using monoclonal antibodies |
| topic | Monoclonal antibodies Antigens B cells Lymphocytes T cells |
| work_keys_str_mv | AT spickettg studiesonthefunctionalheterogeneityofrattcellsusingmonoclonalantibodies AT spickettgp studiesonthefunctionalheterogeneityofrattcellsusingmonoclonalantibodies |