Anaerobic crystallisation of an isopenicillin N synthase.Fe(II).substrate complex demonstrated by X-ray studies.

Anaerobic crystallisation of an isopenicillin N synthase.Fe(II).substrate complex demonstrated by X-ray studies.

Isopenicillin N synthase (IPNS) was cocrystallised with ferrous sulphate and its substrate, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (Aad-Cys-Val). Vital to the successful procedure was the maintenance of a rigorously anaerobic environment. Hanging-drop vapour-diffusion crystallisation expe...

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Bibliographic Details
Main Authors:	Roach, P, Clifton, I, Hensgens, C, Shibata, N, Long, A, Strange, R, Hasnain, S, Schofield, C, Baldwin, J, Hajdu, J
Format:	Journal article
Language:	English
Published:	1996

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