The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
This study tested the hypothesis that a set of predominantly myeloid restricted receptors (F4/80, CD36, Dectin-1, CD200 receptor and mannan binding lectins) and the broadly expressed CD200 played a role in a key function of plasmacytoid DC (pDC), virally induced type I interferon (IFN) production. T...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1826262315021893632 |
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| author | Seeds, R Mukhopadhyay, S Jones, I Gordon, S Miller, J |
| author_facet | Seeds, R Mukhopadhyay, S Jones, I Gordon, S Miller, J |
| author_sort | Seeds, R |
| collection | OXFORD |
| description | This study tested the hypothesis that a set of predominantly myeloid restricted receptors (F4/80, CD36, Dectin-1, CD200 receptor and mannan binding lectins) and the broadly expressed CD200 played a role in a key function of plasmacytoid DC (pDC), virally induced type I interferon (IFN) production. The Dectin-1 ligands zymosan, glucan phosphate and the anti-Dectin-1 monoclonal antibody (mAb) 2A11 had no effect on influenza virus induced IFNα/β production by murine splenic pDC. However, mannan, a broad blocking reagent against mannose specific receptors, inhibited IFNα/β production by pDC in response to inactivated influenza virus. Moreover, viral glycoproteins (influenza virus haemagglutinin and HIV-1 gp120) stimulated IFNα/β production by splenocytes in a mannan-inhibitable manner, implicating the function of a lectin in glycoprotein induced IFN production. Lastly, the effect of CD200 on IFN induction was investigated. CD200 knock-out macrophages produced more IFNα than wild-type macrophages in response to polyI:C, a MyD88-independent stimulus, consistent with CD200's known inhibitory effect on myeloid cells. In contrast, blocking CD200 with an anti-CD200 mAb resulted in reduced IFNα production by pDC-containing splenocytes in response to CpG and influenza virus (MyD88-dependent stimuli). This suggests there could be a differential effect of CD200 on MyD88 dependent and independent IFN induction pathways in pDC and macrophages. This study supports the hypothesis that a mannan-inhibitable lectin and CD200 are involved in virally induced type I IFN induction. |
| first_indexed | 2024-03-06T19:34:28Z |
| format | Journal article |
| id | oxford-uuid:1e9292d2-12d7-4c41-b50e-0b4ab370b3ed |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:34:28Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:1e9292d2-12d7-4c41-b50e-0b4ab370b3ed2022-03-26T11:17:05ZThe role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1e9292d2-12d7-4c41-b50e-0b4ab370b3edEnglishSymplectic Elements at Oxford2011Seeds, RMukhopadhyay, SJones, IGordon, SMiller, JThis study tested the hypothesis that a set of predominantly myeloid restricted receptors (F4/80, CD36, Dectin-1, CD200 receptor and mannan binding lectins) and the broadly expressed CD200 played a role in a key function of plasmacytoid DC (pDC), virally induced type I interferon (IFN) production. The Dectin-1 ligands zymosan, glucan phosphate and the anti-Dectin-1 monoclonal antibody (mAb) 2A11 had no effect on influenza virus induced IFNα/β production by murine splenic pDC. However, mannan, a broad blocking reagent against mannose specific receptors, inhibited IFNα/β production by pDC in response to inactivated influenza virus. Moreover, viral glycoproteins (influenza virus haemagglutinin and HIV-1 gp120) stimulated IFNα/β production by splenocytes in a mannan-inhibitable manner, implicating the function of a lectin in glycoprotein induced IFN production. Lastly, the effect of CD200 on IFN induction was investigated. CD200 knock-out macrophages produced more IFNα than wild-type macrophages in response to polyI:C, a MyD88-independent stimulus, consistent with CD200's known inhibitory effect on myeloid cells. In contrast, blocking CD200 with an anti-CD200 mAb resulted in reduced IFNα production by pDC-containing splenocytes in response to CpG and influenza virus (MyD88-dependent stimuli). This suggests there could be a differential effect of CD200 on MyD88 dependent and independent IFN induction pathways in pDC and macrophages. This study supports the hypothesis that a mannan-inhibitable lectin and CD200 are involved in virally induced type I IFN induction. |
| spellingShingle | Seeds, R Mukhopadhyay, S Jones, I Gordon, S Miller, J The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon. |
| title | The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon. |
| title_full | The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon. |
| title_fullStr | The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon. |
| title_full_unstemmed | The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon. |
| title_short | The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon. |
| title_sort | role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type i interferon |
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