Direct evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones.
The binding of nominal antigen to Ti alpha-beta heterodimers on MHC-restricted human T cell clones specific for fluorescein-5-isothiocyanate (FL) was detected by flow cytometry and affinity chromatography. The FL-Ti interaction is of physiologic significance, since T cell activation is induced by cr...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1986
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| _version_ | 1797057361071833088 |
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| author | Siliciano, R Hemesath, T Pratt, J Dintzis, R Dintzis, H Acuto, O Shin, H Reinherz, E |
| author_facet | Siliciano, R Hemesath, T Pratt, J Dintzis, R Dintzis, H Acuto, O Shin, H Reinherz, E |
| author_sort | Siliciano, R |
| collection | OXFORD |
| description | The binding of nominal antigen to Ti alpha-beta heterodimers on MHC-restricted human T cell clones specific for fluorescein-5-isothiocyanate (FL) was detected by flow cytometry and affinity chromatography. The FL-Ti interaction is of physiologic significance, since T cell activation is induced by cross-linked arrays of FL in the absence of the specific MHC recognition. High antigen valence is required to achieve stable binding to cells and subsequent activation, which is consistent with estimated Ti-FL association constants of less than 3 X 10(5) l/mol. In addition to providing direct evidence that the Ti alpha-beta heterodimer is the receptor for antigen, these data suggest that nominal antigen binding sites exist on the Ti molecules of at least some MHC-restricted clones. |
| first_indexed | 2024-03-06T19:35:12Z |
| format | Journal article |
| id | oxford-uuid:1ecef618-817c-4bf1-b683-f432c50729fb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:35:12Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:1ecef618-817c-4bf1-b683-f432c50729fb2022-03-26T11:18:25ZDirect evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1ecef618-817c-4bf1-b683-f432c50729fbEnglishSymplectic Elements at Oxford1986Siliciano, RHemesath, TPratt, JDintzis, RDintzis, HAcuto, OShin, HReinherz, EThe binding of nominal antigen to Ti alpha-beta heterodimers on MHC-restricted human T cell clones specific for fluorescein-5-isothiocyanate (FL) was detected by flow cytometry and affinity chromatography. The FL-Ti interaction is of physiologic significance, since T cell activation is induced by cross-linked arrays of FL in the absence of the specific MHC recognition. High antigen valence is required to achieve stable binding to cells and subsequent activation, which is consistent with estimated Ti-FL association constants of less than 3 X 10(5) l/mol. In addition to providing direct evidence that the Ti alpha-beta heterodimer is the receptor for antigen, these data suggest that nominal antigen binding sites exist on the Ti molecules of at least some MHC-restricted clones. |
| spellingShingle | Siliciano, R Hemesath, T Pratt, J Dintzis, R Dintzis, H Acuto, O Shin, H Reinherz, E Direct evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones. |
| title | Direct evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones. |
| title_full | Direct evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones. |
| title_fullStr | Direct evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones. |
| title_full_unstemmed | Direct evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones. |
| title_short | Direct evidence for the existence of nominal antigen binding sites on T cell surface Ti alpha-beta heterodimers of MHC-restricted T cell clones. |
| title_sort | direct evidence for the existence of nominal antigen binding sites on t cell surface ti alpha beta heterodimers of mhc restricted t cell clones |
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