Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip.
Quantitative detection of phosphorylation levels is challenging and requires an expertise in both stable isotope labeling as well as enrichment of phosphorylated peptides. Recently, a microfluidic device incorporating a nanoliter flow rate reversed phase column as well as a titania (TiO(2)) enrichme...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1826262367947718656 |
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| author | Polat, A Kraiczek, K Heck, A Raijmakers, R Mohammed, S |
| author_facet | Polat, A Kraiczek, K Heck, A Raijmakers, R Mohammed, S |
| author_sort | Polat, A |
| collection | OXFORD |
| description | Quantitative detection of phosphorylation levels is challenging and requires an expertise in both stable isotope labeling as well as enrichment of phosphorylated peptides. Recently, a microfluidic device incorporating a nanoliter flow rate reversed phase column as well as a titania (TiO(2)) enrichment column was released. This HPLC phosphochip allows excellent recovery and separation of phosphorylated peptides in a robust and reproducible manner with little user intervention. In this work, we have extended the abilities of this chip by defining the conditions required for on-chip stable isotope dimethyl labeling allowing for automated quantitation. The resulting approach will make quantitative phosphoproteomics more accessible. |
| first_indexed | 2024-03-06T19:35:20Z |
| format | Journal article |
| id | oxford-uuid:1ed946d8-8a3e-4117-ae0f-96e7b6404d7a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:35:20Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:1ed946d8-8a3e-4117-ae0f-96e7b6404d7a2022-03-26T11:18:38ZFully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1ed946d8-8a3e-4117-ae0f-96e7b6404d7aEnglishSymplectic Elements at Oxford2012Polat, AKraiczek, KHeck, ARaijmakers, RMohammed, SQuantitative detection of phosphorylation levels is challenging and requires an expertise in both stable isotope labeling as well as enrichment of phosphorylated peptides. Recently, a microfluidic device incorporating a nanoliter flow rate reversed phase column as well as a titania (TiO(2)) enrichment column was released. This HPLC phosphochip allows excellent recovery and separation of phosphorylated peptides in a robust and reproducible manner with little user intervention. In this work, we have extended the abilities of this chip by defining the conditions required for on-chip stable isotope dimethyl labeling allowing for automated quantitation. The resulting approach will make quantitative phosphoproteomics more accessible. |
| spellingShingle | Polat, A Kraiczek, K Heck, A Raijmakers, R Mohammed, S Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip. |
| title | Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip. |
| title_full | Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip. |
| title_fullStr | Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip. |
| title_full_unstemmed | Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip. |
| title_short | Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip. |
| title_sort | fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic hplc phosphochip |
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