Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore
The optimisation of unbiased deep-sequencing methods for full-length HBV genomes will inform detailed insights into the evolution and diversity of this pathogen. Nanopore-based sequencing technologies offer the potential for real-time detection and sequencing of blood-borne viruses, useful for clini...
| Main Authors: | , , , , , , , |
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| Format: | Conference item |
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Elsevier
2018
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| _version_ | 1826262421596012544 |
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| author | Mcnaughton, A Bonsall, D De Cesare, M Parks, D Brown, A Bowden, R Barnes, E Matthews, P |
| author_facet | Mcnaughton, A Bonsall, D De Cesare, M Parks, D Brown, A Bowden, R Barnes, E Matthews, P |
| author_sort | Mcnaughton, A |
| collection | OXFORD |
| description | The optimisation of unbiased deep-sequencing methods for full-length HBV genomes will inform detailed insights into the evolution and diversity of this pathogen. Nanopore-based sequencing technologies offer the potential for real-time detection and sequencing of blood-borne viruses, useful for clinical diagnosis, drug-resistance calling and high-resolution molecular epidemiology. To date, sequencing accuracy has limited the full potential of Oxford Nanopore’s long-read capabilities. We here describe use of an isothermal rolling-circle amplification (RCA) method, (i) to amplify HBV DNA and (ii) to derive concatemers of whole HBV genomes thus providing a mechanism to correct sequencing errors. |
| first_indexed | 2024-03-06T19:35:55Z |
| format | Conference item |
| id | oxford-uuid:1f0a2361-4928-4a6e-b0c1-1a3c7dd4d216 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T19:35:55Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:1f0a2361-4928-4a6e-b0c1-1a3c7dd4d2162022-03-26T11:19:39ZDeep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and NanoporeConference itemhttp://purl.org/coar/resource_type/c_5794uuid:1f0a2361-4928-4a6e-b0c1-1a3c7dd4d216Symplectic Elements at OxfordElsevier2018Mcnaughton, ABonsall, DDe Cesare, MParks, DBrown, ABowden, RBarnes, EMatthews, PThe optimisation of unbiased deep-sequencing methods for full-length HBV genomes will inform detailed insights into the evolution and diversity of this pathogen. Nanopore-based sequencing technologies offer the potential for real-time detection and sequencing of blood-borne viruses, useful for clinical diagnosis, drug-resistance calling and high-resolution molecular epidemiology. To date, sequencing accuracy has limited the full potential of Oxford Nanopore’s long-read capabilities. We here describe use of an isothermal rolling-circle amplification (RCA) method, (i) to amplify HBV DNA and (ii) to derive concatemers of whole HBV genomes thus providing a mechanism to correct sequencing errors. |
| spellingShingle | Mcnaughton, A Bonsall, D De Cesare, M Parks, D Brown, A Bowden, R Barnes, E Matthews, P Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore |
| title | Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore |
| title_full | Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore |
| title_fullStr | Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore |
| title_full_unstemmed | Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore |
| title_short | Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore |
| title_sort | deep sequencing of hepatitis b virus hbv genomes using rolling circle amplification and nanopore |
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