The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells
The mammalian ERCC1-XPF endonuclease has a suggested role in the repair of DNA double-strand breaks (DSB) by single-strand annealing (SSA). Here, we investigated the role of ERCC1 in homologous recombination in mammalian cells, and confirm a role of ERCC1 in SSA. Interestingly, we also report an une...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2008
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| _version_ | 1797057514835017728 |
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| author | Al-Minawi, A Saleh-Gohari, N Helleday, T |
| author_facet | Al-Minawi, A Saleh-Gohari, N Helleday, T |
| author_sort | Al-Minawi, A |
| collection | OXFORD |
| description | The mammalian ERCC1-XPF endonuclease has a suggested role in the repair of DNA double-strand breaks (DSB) by single-strand annealing (SSA). Here, we investigated the role of ERCC1 in homologous recombination in mammalian cells, and confirm a role of ERCC1 in SSA. Interestingly, we also report an unexpected role for ERCC1 in gene conversion. This provides support that gene conversion in mammalian somatic cells is carried out through synthesis-dependent strand annealing, rather than through a double Holliday Junction mechanism. Moreover, we find low frequencies of SSA and gene conversion in G1-arrested cells, suggesting that SSA is not a frequent DSB repair pathway in G1-arrested mammalian cells, even in the presence of perfect repeats. Furthermore, we find that SSA is not influenced by inhibition of CDK2 (using Roscovitine), ATM (using Caffeine and KU55933), Chk1 (using CEP-3891) or DNA-PK (using NU7026). |
| first_indexed | 2024-03-06T19:37:30Z |
| format | Journal article |
| id | oxford-uuid:1f898294-5446-405b-93e7-9e36f533a0c8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:37:30Z |
| publishDate | 2008 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:1f898294-5446-405b-93e7-9e36f533a0c82022-03-26T11:22:26ZThe ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1f898294-5446-405b-93e7-9e36f533a0c8Life SciencesEnglishOxford University Research Archive - ValetOxford University Press2008Al-Minawi, ASaleh-Gohari, NHelleday, TThe mammalian ERCC1-XPF endonuclease has a suggested role in the repair of DNA double-strand breaks (DSB) by single-strand annealing (SSA). Here, we investigated the role of ERCC1 in homologous recombination in mammalian cells, and confirm a role of ERCC1 in SSA. Interestingly, we also report an unexpected role for ERCC1 in gene conversion. This provides support that gene conversion in mammalian somatic cells is carried out through synthesis-dependent strand annealing, rather than through a double Holliday Junction mechanism. Moreover, we find low frequencies of SSA and gene conversion in G1-arrested cells, suggesting that SSA is not a frequent DSB repair pathway in G1-arrested mammalian cells, even in the presence of perfect repeats. Furthermore, we find that SSA is not influenced by inhibition of CDK2 (using Roscovitine), ATM (using Caffeine and KU55933), Chk1 (using CEP-3891) or DNA-PK (using NU7026). |
| spellingShingle | Life Sciences Al-Minawi, A Saleh-Gohari, N Helleday, T The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells |
| title | The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells |
| title_full | The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells |
| title_fullStr | The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells |
| title_full_unstemmed | The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells |
| title_short | The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells |
| title_sort | ercc1 xpf endonuclease is required for efficient single strand annealing and gene conversion in mammalian cells |
| topic | Life Sciences |
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