Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.
The splenic marginal zone is a unique microenvironment where resident immune cells are exposed to the open blood circulation. Even though it has an important role in responses against blood-borne antigens, lymphocyte migration in the marginal zone has not been intravitally visualized due to challeng...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1797057575748894720 |
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| author | Arnon, T Horton, R Grigorova, I Cyster, J |
| author_facet | Arnon, T Horton, R Grigorova, I Cyster, J |
| author_sort | Arnon, T |
| collection | OXFORD |
| description | The splenic marginal zone is a unique microenvironment where resident immune cells are exposed to the open blood circulation. Even though it has an important role in responses against blood-borne antigens, lymphocyte migration in the marginal zone has not been intravitally visualized due to challenges associated with achieving adequate imaging depth in this abdominal organ. Here we develop a two-photon microscopy procedure to study marginal zone and follicular B-cell movement in the live mouse spleen. We show that marginal zone B cells are highly motile and exhibit long membrane extensions. Marginal zone B cells shuttle between the marginal zone and follicles with at least one-fifth of the cells exchanging between compartments per hour, a behaviour that explains their ability to deliver antigens rapidly from the open blood circulation to the secluded follicles. Follicular B cells also transit from follicles to the marginal zone, but unlike marginal zone B cells, they fail to undergo integrin-mediated adhesion, become caught in fluid flow and are carried into the red pulp. Follicular B-cell egress via the marginal zone is sphingosine-1-phosphate receptor-1 (S1PR1)-dependent. This study shows that marginal zone B cells migrate continually between marginal zone and follicles and establishes the marginal zone as a site of S1PR1-dependent B-cell exit from follicles. The results also show how adhesive differences of similar cells critically influence their behaviour in the same microenvironment. |
| first_indexed | 2024-03-06T19:38:24Z |
| format | Journal article |
| id | oxford-uuid:1fcfbde5-a388-4622-8e2d-8673362f7f0a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:38:24Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:1fcfbde5-a388-4622-8e2d-8673362f7f0a2022-03-26T11:24:07ZVisualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1fcfbde5-a388-4622-8e2d-8673362f7f0aEnglishSymplectic Elements at Oxford2013Arnon, THorton, RGrigorova, ICyster, JThe splenic marginal zone is a unique microenvironment where resident immune cells are exposed to the open blood circulation. Even though it has an important role in responses against blood-borne antigens, lymphocyte migration in the marginal zone has not been intravitally visualized due to challenges associated with achieving adequate imaging depth in this abdominal organ. Here we develop a two-photon microscopy procedure to study marginal zone and follicular B-cell movement in the live mouse spleen. We show that marginal zone B cells are highly motile and exhibit long membrane extensions. Marginal zone B cells shuttle between the marginal zone and follicles with at least one-fifth of the cells exchanging between compartments per hour, a behaviour that explains their ability to deliver antigens rapidly from the open blood circulation to the secluded follicles. Follicular B cells also transit from follicles to the marginal zone, but unlike marginal zone B cells, they fail to undergo integrin-mediated adhesion, become caught in fluid flow and are carried into the red pulp. Follicular B-cell egress via the marginal zone is sphingosine-1-phosphate receptor-1 (S1PR1)-dependent. This study shows that marginal zone B cells migrate continually between marginal zone and follicles and establishes the marginal zone as a site of S1PR1-dependent B-cell exit from follicles. The results also show how adhesive differences of similar cells critically influence their behaviour in the same microenvironment. |
| spellingShingle | Arnon, T Horton, R Grigorova, I Cyster, J Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. |
| title | Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. |
| title_full | Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. |
| title_fullStr | Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. |
| title_full_unstemmed | Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. |
| title_short | Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. |
| title_sort | visualization of splenic marginal zone b cell shuttling and follicular b cell egress |
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