EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS
Purification and sequencing of clavaminic acid synthase (CAS) activities from S. clavuligerus SC2 provided evidence for the existence of two CAS isozymes. One of these isozymes was cloned and expressed in E. coli. The recombinant CAS isozyme was shown to catalyse the hydroxylation of (2S)-5-guanidin...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1994
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| _version_ | 1797057582858240000 |
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| author | Lawlor, E Elson, S Holland, S Cassels, R Hodgson, J Lloyd, M Baldwin, J Schofield, C |
| author_facet | Lawlor, E Elson, S Holland, S Cassels, R Hodgson, J Lloyd, M Baldwin, J Schofield, C |
| author_sort | Lawlor, E |
| collection | OXFORD |
| description | Purification and sequencing of clavaminic acid synthase (CAS) activities from S. clavuligerus SC2 provided evidence for the existence of two CAS isozymes. One of these isozymes was cloned and expressed in E. coli. The recombinant CAS isozyme was shown to catalyse the hydroxylation of (2S)-5-guanidino-2-(2′-oxoazetidin-1′-yl)pentanoic acid, in addition to the production of clavaminic acid from dihydroclavaminic acid via proclavaminic acid, consistent with the trifunctional role proposed for clavaminic acid synthase in the biosynthesis of clavulanic acid. A preliminary purification and characterisation of recombinant isozyme is reported. © 1994. |
| first_indexed | 2024-03-06T19:38:31Z |
| format | Journal article |
| id | oxford-uuid:1fda5b84-5061-42d0-8512-5252308eb117 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:38:31Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:1fda5b84-5061-42d0-8512-5252308eb1172022-03-26T11:24:21ZEXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESISJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1fda5b84-5061-42d0-8512-5252308eb117EnglishSymplectic Elements at Oxford1994Lawlor, EElson, SHolland, SCassels, RHodgson, JLloyd, MBaldwin, JSchofield, CPurification and sequencing of clavaminic acid synthase (CAS) activities from S. clavuligerus SC2 provided evidence for the existence of two CAS isozymes. One of these isozymes was cloned and expressed in E. coli. The recombinant CAS isozyme was shown to catalyse the hydroxylation of (2S)-5-guanidino-2-(2′-oxoazetidin-1′-yl)pentanoic acid, in addition to the production of clavaminic acid from dihydroclavaminic acid via proclavaminic acid, consistent with the trifunctional role proposed for clavaminic acid synthase in the biosynthesis of clavulanic acid. A preliminary purification and characterisation of recombinant isozyme is reported. © 1994. |
| spellingShingle | Lawlor, E Elson, S Holland, S Cassels, R Hodgson, J Lloyd, M Baldwin, J Schofield, C EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS |
| title | EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS |
| title_full | EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS |
| title_fullStr | EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS |
| title_full_unstemmed | EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS |
| title_short | EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS |
| title_sort | expression in escherichia coli of a clavaminic acid synthase isozyme a trifunctional oxygenase involved in clavulanic acid biosynthesis |
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