Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver
Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Public Library of Science
2017
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| _version_ | 1797057595522940928 |
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| author | Nagarajan, SR Brandon, AE McKenna, JA Shtein, HC Nguyen, TQ Suryana, E Poronnik, P Cooney, GJ Saunders, DS Hoy, AJ |
| author_facet | Nagarajan, SR Brandon, AE McKenna, JA Shtein, HC Nguyen, TQ Suryana, E Poronnik, P Cooney, GJ Saunders, DS Hoy, AJ |
| author_sort | Nagarajan, SR |
| collection | OXFORD |
| description | Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance. |
| first_indexed | 2024-03-06T19:38:37Z |
| format | Journal article |
| id | oxford-uuid:1fe2b3e3-1ece-49dd-bdb0-12dde20d6080 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:38:37Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:1fe2b3e3-1ece-49dd-bdb0-12dde20d60802022-03-26T11:24:38ZInsulin and diet-induced changes in the ubiquitin-modified proteome of rat liverJournal articlehttp://purl.org/coar/resource_type/c_6501uuid:1fe2b3e3-1ece-49dd-bdb0-12dde20d6080EnglishSymplectic ElementsPublic Library of Science2017Nagarajan, SRBrandon, AEMcKenna, JAShtein, HCNguyen, TQSuryana, EPoronnik, PCooney, GJSaunders, DSHoy, AJUbiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance. |
| spellingShingle | Nagarajan, SR Brandon, AE McKenna, JA Shtein, HC Nguyen, TQ Suryana, E Poronnik, P Cooney, GJ Saunders, DS Hoy, AJ Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver |
| title | Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver |
| title_full | Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver |
| title_fullStr | Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver |
| title_full_unstemmed | Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver |
| title_short | Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver |
| title_sort | insulin and diet induced changes in the ubiquitin modified proteome of rat liver |
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