Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II.
The requirement of poly(A) signals to elicit transcription termination of RNA polymerase II (pol II) is firmly established. However, little else is known about the actual process of pol II transcription termination. Evidence presented in this paper, based on analysis of nascent transcripts of the hu...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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2001
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| _version_ | 1826262689043709952 |
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| author | Dye, M Proudfoot, N |
| author_facet | Dye, M Proudfoot, N |
| author_sort | Dye, M |
| collection | OXFORD |
| description | The requirement of poly(A) signals to elicit transcription termination of RNA polymerase II (pol II) is firmly established. However, little else is known about the actual process of pol II transcription termination. Evidence presented in this paper, based on analysis of nascent transcripts of the human beta- and epsilon-globin genes, demonstrates that pol II transcription termination involves two distinct and temporally separate events. The first event, termed pretermination cleavage (PTC), is mediated by sequence tracts located downstream of the poly(A) site which appear to promote heterogeneous cleavage of the nascent transcript. The second event, in which pol II disengages from the DNA template, requires that polymerase has transcribed both a PTC sequence tract and a functional poly(A) site. |
| first_indexed | 2024-03-06T19:40:02Z |
| format | Journal article |
| id | oxford-uuid:205841a7-6b90-4a95-9389-93391f5534d5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:40:02Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:205841a7-6b90-4a95-9389-93391f5534d52022-03-26T11:27:06ZMultiple transcript cleavage precedes polymerase release in termination by RNA polymerase II.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:205841a7-6b90-4a95-9389-93391f5534d5EnglishSymplectic Elements at Oxford2001Dye, MProudfoot, NThe requirement of poly(A) signals to elicit transcription termination of RNA polymerase II (pol II) is firmly established. However, little else is known about the actual process of pol II transcription termination. Evidence presented in this paper, based on analysis of nascent transcripts of the human beta- and epsilon-globin genes, demonstrates that pol II transcription termination involves two distinct and temporally separate events. The first event, termed pretermination cleavage (PTC), is mediated by sequence tracts located downstream of the poly(A) site which appear to promote heterogeneous cleavage of the nascent transcript. The second event, in which pol II disengages from the DNA template, requires that polymerase has transcribed both a PTC sequence tract and a functional poly(A) site. |
| spellingShingle | Dye, M Proudfoot, N Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. |
| title | Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. |
| title_full | Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. |
| title_fullStr | Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. |
| title_full_unstemmed | Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. |
| title_short | Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. |
| title_sort | multiple transcript cleavage precedes polymerase release in termination by rna polymerase ii |
| work_keys_str_mv | AT dyem multipletranscriptcleavageprecedespolymerasereleaseinterminationbyrnapolymeraseii AT proudfootn multipletranscriptcleavageprecedespolymerasereleaseinterminationbyrnapolymeraseii |