Two-state model of conformational fluctuation in a DNA hairpin-loop
Stretched exponential kinetics have been observed in the conformational fluctuation of a DNA hairpin-loop under equilibrium conditions. In this paper, we employ a simple multiple-pathway two-state jump model to calculate single-molecule proximity ratio distributions. The simulation can reasonably re...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2001
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| _version_ | 1826262694874841088 |
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| author | Ying, L Wallace, M Klenerman, D |
| author_facet | Ying, L Wallace, M Klenerman, D |
| author_sort | Ying, L |
| collection | OXFORD |
| description | Stretched exponential kinetics have been observed in the conformational fluctuation of a DNA hairpin-loop under equilibrium conditions. In this paper, we employ a simple multiple-pathway two-state jump model to calculate single-molecule proximity ratio distributions. The simulation can reasonably reproduce the experimental single-molecule data of the conformational fluctuations in water, indicating that static disorder is dominant. In contrast, there exists significant discrepancy between the two-state simulation and experiment in buffer (2.5 mM Tris-HCl, 250μM EDTA, 100 mM NaCl), suggesting that both static and dynamic disorder may contribute to the non-exponential kinetics. © 2001 Elsevier Science B.V. |
| first_indexed | 2024-03-06T19:40:08Z |
| format | Journal article |
| id | oxford-uuid:20622d92-b87f-427b-b2ca-e80b35a5911d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:40:08Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:20622d92-b87f-427b-b2ca-e80b35a5911d2022-03-26T11:27:19ZTwo-state model of conformational fluctuation in a DNA hairpin-loopJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:20622d92-b87f-427b-b2ca-e80b35a5911dEnglishSymplectic Elements at Oxford2001Ying, LWallace, MKlenerman, DStretched exponential kinetics have been observed in the conformational fluctuation of a DNA hairpin-loop under equilibrium conditions. In this paper, we employ a simple multiple-pathway two-state jump model to calculate single-molecule proximity ratio distributions. The simulation can reasonably reproduce the experimental single-molecule data of the conformational fluctuations in water, indicating that static disorder is dominant. In contrast, there exists significant discrepancy between the two-state simulation and experiment in buffer (2.5 mM Tris-HCl, 250μM EDTA, 100 mM NaCl), suggesting that both static and dynamic disorder may contribute to the non-exponential kinetics. © 2001 Elsevier Science B.V. |
| spellingShingle | Ying, L Wallace, M Klenerman, D Two-state model of conformational fluctuation in a DNA hairpin-loop |
| title | Two-state model of conformational fluctuation in a DNA hairpin-loop |
| title_full | Two-state model of conformational fluctuation in a DNA hairpin-loop |
| title_fullStr | Two-state model of conformational fluctuation in a DNA hairpin-loop |
| title_full_unstemmed | Two-state model of conformational fluctuation in a DNA hairpin-loop |
| title_short | Two-state model of conformational fluctuation in a DNA hairpin-loop |
| title_sort | two state model of conformational fluctuation in a dna hairpin loop |
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