PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis.
PCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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| _version_ | 1826262787832152064 |
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| author | Caws, M Tho, D Duy, P Lan, N Hoa, D Torok, M Chau, T Chau, N Chinh, N Farrar, J |
| author_facet | Caws, M Tho, D Duy, P Lan, N Hoa, D Torok, M Chau, T Chau, N Chinh, N Farrar, J |
| author_sort | Caws, M |
| collection | OXFORD |
| description | PCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-resistant katG_315 mutants. The test had a sensitivity of 80% and a specificity of 100% against conventional phenotypic drug susceptibility testing. The positive and negative predictive values were 1 and 0.86, respectively. None of the discrepant isolates had mutant katG_315 codons by sequencing. The test is cheap (less than $1.50 per test), specific, and suitable for the rapid identification of isoniazid resistance in regions with a high prevalence of katG_315 mutants among isoniazid-resistant M. tuberculosis isolates. |
| first_indexed | 2024-03-06T19:41:37Z |
| format | Journal article |
| id | oxford-uuid:20e04f86-b107-426e-9d6b-77e4e2d84857 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:41:37Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:20e04f86-b107-426e-9d6b-77e4e2d848572022-03-26T11:29:58ZPCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:20e04f86-b107-426e-9d6b-77e4e2d84857EnglishSymplectic Elements at Oxford2007Caws, MTho, DDuy, PLan, NHoa, DTorok, MChau, TChau, NChinh, NFarrar, JPCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-resistant katG_315 mutants. The test had a sensitivity of 80% and a specificity of 100% against conventional phenotypic drug susceptibility testing. The positive and negative predictive values were 1 and 0.86, respectively. None of the discrepant isolates had mutant katG_315 codons by sequencing. The test is cheap (less than $1.50 per test), specific, and suitable for the rapid identification of isoniazid resistance in regions with a high prevalence of katG_315 mutants among isoniazid-resistant M. tuberculosis isolates. |
| spellingShingle | Caws, M Tho, D Duy, P Lan, N Hoa, D Torok, M Chau, T Chau, N Chinh, N Farrar, J PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. |
| title | PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. |
| title_full | PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. |
| title_fullStr | PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. |
| title_full_unstemmed | PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. |
| title_short | PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. |
| title_sort | pcr restriction fragment length polymorphism for rapid low cost identification of isoniazid resistant mycobacterium tuberculosis |
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