Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry.
Hydrophilic interaction liquid chromatography (HILIC) is here successfully coupled to negative-ion electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) for the analysis of synthetic and chemically modified oligonucleotides. Separation was performed on a 2.1 mm × 100 mm PEEK ZIC HILIC...
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1797057920320405504 |
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| author | Gong, L McCullagh, J |
| author_facet | Gong, L McCullagh, J |
| author_sort | Gong, L |
| collection | OXFORD |
| description | Hydrophilic interaction liquid chromatography (HILIC) is here successfully coupled to negative-ion electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) for the analysis of synthetic and chemically modified oligonucleotides. Separation was performed on a 2.1 mm × 100 mm PEEK ZIC HILIC column packed with hydrophilic stationary phase with a permanent zwitterionic functional group and a particle size of 3.5 μm with an average pore diameter of 200Å. A method was developed to separate homogeneous and heterogeneous oligonucleotides as well as methylated oligonucleotides using a quaternary pumping system containing ammonium acetate and water with an acetonitrile gradient. Analyses of oligonucleotides were performed by LC/MS with a detection limit of 2.5 picomole (20 mer) with signal to noise ratio (S/N) of 4.12. The influence of the eluent composition, type of buffer and its concentration, and organic modifier were also evaluated. The HILIC LC/MS method presented in this paper used common, 'MS friendly', mobile phases achieving sensitive and selective oligonucleotide analysis. |
| first_indexed | 2024-03-06T19:43:11Z |
| format | Journal article |
| id | oxford-uuid:215d42fb-a59f-45a5-9c78-5abc5b6b63f6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:43:11Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:215d42fb-a59f-45a5-9c78-5abc5b6b63f62022-03-26T11:33:00ZAnalysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:215d42fb-a59f-45a5-9c78-5abc5b6b63f6EnglishSymplectic Elements at Oxford2011Gong, LMcCullagh, JHydrophilic interaction liquid chromatography (HILIC) is here successfully coupled to negative-ion electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) for the analysis of synthetic and chemically modified oligonucleotides. Separation was performed on a 2.1 mm × 100 mm PEEK ZIC HILIC column packed with hydrophilic stationary phase with a permanent zwitterionic functional group and a particle size of 3.5 μm with an average pore diameter of 200Å. A method was developed to separate homogeneous and heterogeneous oligonucleotides as well as methylated oligonucleotides using a quaternary pumping system containing ammonium acetate and water with an acetonitrile gradient. Analyses of oligonucleotides were performed by LC/MS with a detection limit of 2.5 picomole (20 mer) with signal to noise ratio (S/N) of 4.12. The influence of the eluent composition, type of buffer and its concentration, and organic modifier were also evaluated. The HILIC LC/MS method presented in this paper used common, 'MS friendly', mobile phases achieving sensitive and selective oligonucleotide analysis. |
| spellingShingle | Gong, L McCullagh, J Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry. |
| title | Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry. |
| title_full | Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry. |
| title_fullStr | Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry. |
| title_full_unstemmed | Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry. |
| title_short | Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry. |
| title_sort | analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry |
| work_keys_str_mv | AT gongl analysisofoligonucleotidesbyhydrophilicinteractionliquidchromatographycoupledtonegativeionelectrosprayionizationmassspectrometry AT mccullaghj analysisofoligonucleotidesbyhydrophilicinteractionliquidchromatographycoupledtonegativeionelectrosprayionizationmassspectrometry |