Nanomedicine: Engineering of a tri-imageable nanoparticle for diagnostics
We have created a potential targeted drug delivery platform with three imaging reporters by coupling the magnetic properties of USPIOs with near infrared fluorescence of Cy5.5 and γ-emissions of 111In that is chelated to a conjugated antibody. The nanoparticle will allow for not only triple verifica...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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| Summary: | We have created a potential targeted drug delivery platform with three imaging reporters by coupling the magnetic properties of USPIOs with near infrared fluorescence of Cy5.5 and γ-emissions of 111In that is chelated to a conjugated antibody. The nanoparticle will allow for not only triple verification of localization, but also quantification. During each phase of development, the nanoparticles have been characterized for surface charge and structure by transmission electron microscopy and dynamic light scattering. Magnetic properties including hysteresis measurements and field cooling analyses were conducted using a superconducting quantum interference device. In vitro analyses of flow cytometry and cell viability as well as in vivo imaging studies have been conducted. |
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