Inhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments.
The efficient binding and inhibition of α-chymotrypsin (ChT) by a self-assembled DNA quadruplex with protein recognition fragments appended on the 5'-ends of the assembled G-quartet was reported. The peptide loops were constructed such that they are broadly complementary to the cationic and hyd...
| Príomhchruthaitheoirí: | , , |
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| Formáid: | Journal article |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
2009
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| _version_ | 1826262931553124352 |
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| author | Cai, J Rosenzweig, B Hamilton, A |
| author_facet | Cai, J Rosenzweig, B Hamilton, A |
| author_sort | Cai, J |
| collection | OXFORD |
| description | The efficient binding and inhibition of α-chymotrypsin (ChT) by a self-assembled DNA quadruplex with protein recognition fragments appended on the 5'-ends of the assembled G-quartet was reported. The peptide loops were constructed such that they are broadly complementary to the cationic and hydrophobic active site region of ChT. The single-strand peptide loop adduct presents only a single monomeric peptide loop to the protein and displays the weakest inhibition. The factorial velocities for the hydrolysis of N-benzoyl tyrosine p-nitroanilide by ChT as a function of preincubation time for different concentrations show a two-step mechanism of inhibition. The protein fragment is also found to display the highest inhibition potency for ChT and also found to be a good inhibitor. |
| first_indexed | 2024-03-06T19:43:38Z |
| format | Journal article |
| id | oxford-uuid:2181de28-eef3-4c80-a9cf-20ff1079636f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:43:38Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:2181de28-eef3-4c80-a9cf-20ff1079636f2022-03-26T11:33:53ZInhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2181de28-eef3-4c80-a9cf-20ff1079636fEnglishSymplectic Elements at Oxford2009Cai, JRosenzweig, BHamilton, AThe efficient binding and inhibition of α-chymotrypsin (ChT) by a self-assembled DNA quadruplex with protein recognition fragments appended on the 5'-ends of the assembled G-quartet was reported. The peptide loops were constructed such that they are broadly complementary to the cationic and hydrophobic active site region of ChT. The single-strand peptide loop adduct presents only a single monomeric peptide loop to the protein and displays the weakest inhibition. The factorial velocities for the hydrolysis of N-benzoyl tyrosine p-nitroanilide by ChT as a function of preincubation time for different concentrations show a two-step mechanism of inhibition. The protein fragment is also found to display the highest inhibition potency for ChT and also found to be a good inhibitor. |
| spellingShingle | Cai, J Rosenzweig, B Hamilton, A Inhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments. |
| title | Inhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments. |
| title_full | Inhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments. |
| title_fullStr | Inhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments. |
| title_full_unstemmed | Inhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments. |
| title_short | Inhibition of chymotrypsin by a self-assembled DNA quadruplex functionalized with cyclic peptide binding fragments. |
| title_sort | inhibition of chymotrypsin by a self assembled dna quadruplex functionalized with cyclic peptide binding fragments |
| work_keys_str_mv | AT caij inhibitionofchymotrypsinbyaselfassembleddnaquadruplexfunctionalizedwithcyclicpeptidebindingfragments AT rosenzweigb inhibitionofchymotrypsinbyaselfassembleddnaquadruplexfunctionalizedwithcyclicpeptidebindingfragments AT hamiltona inhibitionofchymotrypsinbyaselfassembleddnaquadruplexfunctionalizedwithcyclicpeptidebindingfragments |