An optimized set of human telomere clones for studying telomere integrity and architecture
Telomere-specific clones are a valuable resource for the characterization of chromosomal rearrangements. We previously reported a first-generation set of human telomere probes consisting of 34 genomic clones, which were a known distance from the end of the chromosome (~300 kb), and 7 clones correspo...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal article |
| Language: | English |
| Published: |
University of Chicago Press
2000
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| _version_ | 1797057994246062080 |
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| author | Knight, S Lese, C Precht, K Kuc, J Ning, Y Lucas, S Regan, R Brenan, M Nicod, A Lawrie, N Cardy, D Nguyen, H Hudson, T Riethman, H Ledbetter, D Flint, J |
| author2 | American Society of Human Genetics |
| author_facet | American Society of Human Genetics Knight, S Lese, C Precht, K Kuc, J Ning, Y Lucas, S Regan, R Brenan, M Nicod, A Lawrie, N Cardy, D Nguyen, H Hudson, T Riethman, H Ledbetter, D Flint, J |
| author_sort | Knight, S |
| collection | OXFORD |
| description | Telomere-specific clones are a valuable resource for the characterization of chromosomal rearrangements. We previously reported a first-generation set of human telomere probes consisting of 34 genomic clones, which were a known distance from the end of the chromosome (~300 kb), and 7 clones corresponding to the most distal markers on the integrated genetic/physical map (1p, 5p, 6p, 9p, 12p, 15q, and 20q). Subsequently, this resource has been optimized and completed: the size of the genomic clones has been expanded to a target size of 100-200 kb, which is optimal for use in genome-scanning methodologies, and additional probes for the remaining seven telomeres have been identified. For each clone we give an associated mapped sequence-tagged site and provide distances from the telomere estimated using a combination of fiberFISH, interphase FISH, sequence analysis, and radiation-hybrid mapping. This updated set of telomeric clones is an invaluable resource for clinical diagnosis and represents an important contribution to genetic and physical mapping efforts aimed at telomeric regions. |
| first_indexed | 2024-03-06T19:44:18Z |
| format | Journal article |
| id | oxford-uuid:21bd4137-3e30-450d-95b7-8459a40c2485 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:44:18Z |
| publishDate | 2000 |
| publisher | University of Chicago Press |
| record_format | dspace |
| spelling | oxford-uuid:21bd4137-3e30-450d-95b7-8459a40c24852022-03-26T11:35:07ZAn optimized set of human telomere clones for studying telomere integrity and architectureJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:21bd4137-3e30-450d-95b7-8459a40c2485Genetics (medical sciences)EnglishOxford University Research Archive - ValetUniversity of Chicago Press2000Knight, SLese, CPrecht, KKuc, JNing, YLucas, SRegan, RBrenan, MNicod, ALawrie, NCardy, DNguyen, HHudson, TRiethman, HLedbetter, DFlint, JAmerican Society of Human GeneticsTelomere-specific clones are a valuable resource for the characterization of chromosomal rearrangements. We previously reported a first-generation set of human telomere probes consisting of 34 genomic clones, which were a known distance from the end of the chromosome (~300 kb), and 7 clones corresponding to the most distal markers on the integrated genetic/physical map (1p, 5p, 6p, 9p, 12p, 15q, and 20q). Subsequently, this resource has been optimized and completed: the size of the genomic clones has been expanded to a target size of 100-200 kb, which is optimal for use in genome-scanning methodologies, and additional probes for the remaining seven telomeres have been identified. For each clone we give an associated mapped sequence-tagged site and provide distances from the telomere estimated using a combination of fiberFISH, interphase FISH, sequence analysis, and radiation-hybrid mapping. This updated set of telomeric clones is an invaluable resource for clinical diagnosis and represents an important contribution to genetic and physical mapping efforts aimed at telomeric regions. |
| spellingShingle | Genetics (medical sciences) Knight, S Lese, C Precht, K Kuc, J Ning, Y Lucas, S Regan, R Brenan, M Nicod, A Lawrie, N Cardy, D Nguyen, H Hudson, T Riethman, H Ledbetter, D Flint, J An optimized set of human telomere clones for studying telomere integrity and architecture |
| title | An optimized set of human telomere clones for studying telomere integrity and architecture |
| title_full | An optimized set of human telomere clones for studying telomere integrity and architecture |
| title_fullStr | An optimized set of human telomere clones for studying telomere integrity and architecture |
| title_full_unstemmed | An optimized set of human telomere clones for studying telomere integrity and architecture |
| title_short | An optimized set of human telomere clones for studying telomere integrity and architecture |
| title_sort | optimized set of human telomere clones for studying telomere integrity and architecture |
| topic | Genetics (medical sciences) |
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