Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists.
Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists,...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1997
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| _version_ | 1826263005609852928 |
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| author | Bachmann, M Mariathasan, S Bouchard, D Speiser, D Ohashi, P |
| author_facet | Bachmann, M Mariathasan, S Bouchard, D Speiser, D Ohashi, P |
| author_sort | Bachmann, M |
| collection | OXFORD |
| description | Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Four different types of Ca2+ responses could be observed. Full agonists triggered a strong and sustained increase in [Ca2+]i. In contrast, partial T cell agonists induced either a strong but transient Ca2+ flux or very low to no increases in [Ca2+]i, while T cell antagonists failed to induce any measurable Ca2+ flux. The ability of peptides to induce elevated [Ca2+]i perfectly paralleled their ability to trigger TCR internalization and T cell activation. Thus, stimulation of naive cytotoxic T cells with a panel of defined altered peptide ligands reveals a consistent picture, where Ca2+ fluxes predict agonist, partial agonist and antagonist properties of peptides. |
| first_indexed | 2024-03-06T19:44:49Z |
| format | Journal article |
| id | oxford-uuid:21ed7ff4-63ef-4192-90a3-ccd13203f60a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:44:49Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:21ed7ff4-63ef-4192-90a3-ccd13203f60a2022-03-26T11:35:56ZFour types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:21ed7ff4-63ef-4192-90a3-ccd13203f60aEnglishSymplectic Elements at Oxford1997Bachmann, MMariathasan, SBouchard, DSpeiser, DOhashi, PStimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Four different types of Ca2+ responses could be observed. Full agonists triggered a strong and sustained increase in [Ca2+]i. In contrast, partial T cell agonists induced either a strong but transient Ca2+ flux or very low to no increases in [Ca2+]i, while T cell antagonists failed to induce any measurable Ca2+ flux. The ability of peptides to induce elevated [Ca2+]i perfectly paralleled their ability to trigger TCR internalization and T cell activation. Thus, stimulation of naive cytotoxic T cells with a panel of defined altered peptide ligands reveals a consistent picture, where Ca2+ fluxes predict agonist, partial agonist and antagonist properties of peptides. |
| spellingShingle | Bachmann, M Mariathasan, S Bouchard, D Speiser, D Ohashi, P Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists. |
| title | Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists. |
| title_full | Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists. |
| title_fullStr | Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists. |
| title_full_unstemmed | Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists. |
| title_short | Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists. |
| title_sort | four types of ca2 signals in naive cd8 cytotoxic t cells after stimulation with t cell agonists partial agonists and antagonists |
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