A protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice.
Whole-genome genetic association studies in outbred mouse populations represent a novel approach to identifying the molecular basis of naturally occurring genetic variants, the major source of quantitative variation between inbred strains of mice. Measuring multiple phenotypes in parallel on each mo...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2006
|
| _version_ | 1797058157499908096 |
|---|---|
| author | Solberg, L Valdar, W Gauguier, D Nunez, G Taylor, A Burnett, S Arboledas-Hita, C Hernandez-Pliego, P Davidson, S Burns, P Bhattacharya, S Hough, T Higgs, D Klenerman, P Cookson, W Zhang, Y Deacon, R Rawlins, J Mott, R Flint, J |
| author_facet | Solberg, L Valdar, W Gauguier, D Nunez, G Taylor, A Burnett, S Arboledas-Hita, C Hernandez-Pliego, P Davidson, S Burns, P Bhattacharya, S Hough, T Higgs, D Klenerman, P Cookson, W Zhang, Y Deacon, R Rawlins, J Mott, R Flint, J |
| author_sort | Solberg, L |
| collection | OXFORD |
| description | Whole-genome genetic association studies in outbred mouse populations represent a novel approach to identifying the molecular basis of naturally occurring genetic variants, the major source of quantitative variation between inbred strains of mice. Measuring multiple phenotypes in parallel on each mouse would make the approach cost effective, but protocols for phenotyping on a large enough scale have not been developed. In this article we describe the development and deployment of a protocol to collect measures on three models of human disease (anxiety, type II diabetes, and asthma) as well as measures of mouse blood biochemistry, immunology, and hematology. We report that the protocol delivers highly significant differences among the eight inbred strains (A/J, AKR/J, BALBc/J, CBA/J, C3H/HeJ, C57BL/6 J, DBA/2 J, and LP/J), the progenitors of a genetically heterogeneous stock (HS) of mice. We report the successful collection of multiple phenotypes from 2000 outbred HS animals. The phenotypes measured in the protocol form the basis of a large-scale investigation into the genetic basis of complex traits in mice designed to examine interactions between genes and between genes and environment, as well as the main effects of genetic variants on phenotypes. |
| first_indexed | 2024-03-06T19:46:35Z |
| format | Journal article |
| id | oxford-uuid:2280652b-1cf6-4db2-bf05-cdbf18f7b482 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:46:35Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:2280652b-1cf6-4db2-bf05-cdbf18f7b4822022-03-26T11:39:12ZA protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2280652b-1cf6-4db2-bf05-cdbf18f7b482EnglishSymplectic Elements at Oxford2006Solberg, LValdar, WGauguier, DNunez, GTaylor, ABurnett, SArboledas-Hita, CHernandez-Pliego, PDavidson, SBurns, PBhattacharya, SHough, THiggs, DKlenerman, PCookson, WZhang, YDeacon, RRawlins, JMott, RFlint, JWhole-genome genetic association studies in outbred mouse populations represent a novel approach to identifying the molecular basis of naturally occurring genetic variants, the major source of quantitative variation between inbred strains of mice. Measuring multiple phenotypes in parallel on each mouse would make the approach cost effective, but protocols for phenotyping on a large enough scale have not been developed. In this article we describe the development and deployment of a protocol to collect measures on three models of human disease (anxiety, type II diabetes, and asthma) as well as measures of mouse blood biochemistry, immunology, and hematology. We report that the protocol delivers highly significant differences among the eight inbred strains (A/J, AKR/J, BALBc/J, CBA/J, C3H/HeJ, C57BL/6 J, DBA/2 J, and LP/J), the progenitors of a genetically heterogeneous stock (HS) of mice. We report the successful collection of multiple phenotypes from 2000 outbred HS animals. The phenotypes measured in the protocol form the basis of a large-scale investigation into the genetic basis of complex traits in mice designed to examine interactions between genes and between genes and environment, as well as the main effects of genetic variants on phenotypes. |
| spellingShingle | Solberg, L Valdar, W Gauguier, D Nunez, G Taylor, A Burnett, S Arboledas-Hita, C Hernandez-Pliego, P Davidson, S Burns, P Bhattacharya, S Hough, T Higgs, D Klenerman, P Cookson, W Zhang, Y Deacon, R Rawlins, J Mott, R Flint, J A protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice. |
| title | A protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice. |
| title_full | A protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice. |
| title_fullStr | A protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice. |
| title_full_unstemmed | A protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice. |
| title_short | A protocol for high-throughput phenotyping, suitable for quantitative trait analysis in mice. |
| title_sort | protocol for high throughput phenotyping suitable for quantitative trait analysis in mice |
| work_keys_str_mv | AT solbergl aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT valdarw aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT gauguierd aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT nunezg aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT taylora aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT burnetts aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT arboledashitac aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT hernandezpliegop aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT davidsons aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT burnsp aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT bhattacharyas aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT hought aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT higgsd aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT klenermanp aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT cooksonw aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT zhangy aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT deaconr aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT rawlinsj aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT mottr aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT flintj aprotocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT solbergl protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT valdarw protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT gauguierd protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT nunezg protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT taylora protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT burnetts protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT arboledashitac protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT hernandezpliegop protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT davidsons protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT burnsp protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT bhattacharyas protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT hought protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT higgsd protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT klenermanp protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT cooksonw protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT zhangy protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT deaconr protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT rawlinsj protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT mottr protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice AT flintj protocolforhighthroughputphenotypingsuitableforquantitativetraitanalysisinmice |