Similar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis.
We have utilized a new radioimmunoassay for rat growth hormone-releasing hormone (GHRH) to investigate the presence of GHRH in different organ systems of adult rat, and specifically the rat central nervous system (CNS). The highest concentration of GHRH was found, as expected, in the hypothalamus, b...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1991
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| _version_ | 1797058160534487040 |
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| author | Tsagarakis, S Ge, F Besser, G Grossman, A |
| author_facet | Tsagarakis, S Ge, F Besser, G Grossman, A |
| author_sort | Tsagarakis, S |
| collection | OXFORD |
| description | We have utilized a new radioimmunoassay for rat growth hormone-releasing hormone (GHRH) to investigate the presence of GHRH in different organ systems of adult rat, and specifically the rat central nervous system (CNS). The highest concentration of GHRH was found, as expected, in the hypothalamus, but significant amounts were also located in the brain cortex, predominantly the frontal cortex, as well as in the testis. Smaller amounts were identified in the cerebellum and brain stem. Sephadex and reversed phase high performance liquid chromatography demonstrated that while hypothalamic GHRH exclusively eluted at the position of rat GHRH (1-43), in testis and brain the major form was predominantly (testis) or wholly (brain) of a higher molecular weight. While this molecular species has yet to be further characterized, the data suggest the similar GHRH-like species exist in the CNS as well as the testis. |
| first_indexed | 2024-03-06T19:46:38Z |
| format | Journal article |
| id | oxford-uuid:2282c3d5-6e08-4aad-9ac9-0a2ea51ee4d4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:46:38Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:2282c3d5-6e08-4aad-9ac9-0a2ea51ee4d42022-03-26T11:39:16ZSimilar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2282c3d5-6e08-4aad-9ac9-0a2ea51ee4d4EnglishSymplectic Elements at Oxford1991Tsagarakis, SGe, FBesser, GGrossman, AWe have utilized a new radioimmunoassay for rat growth hormone-releasing hormone (GHRH) to investigate the presence of GHRH in different organ systems of adult rat, and specifically the rat central nervous system (CNS). The highest concentration of GHRH was found, as expected, in the hypothalamus, but significant amounts were also located in the brain cortex, predominantly the frontal cortex, as well as in the testis. Smaller amounts were identified in the cerebellum and brain stem. Sephadex and reversed phase high performance liquid chromatography demonstrated that while hypothalamic GHRH exclusively eluted at the position of rat GHRH (1-43), in testis and brain the major form was predominantly (testis) or wholly (brain) of a higher molecular weight. While this molecular species has yet to be further characterized, the data suggest the similar GHRH-like species exist in the CNS as well as the testis. |
| spellingShingle | Tsagarakis, S Ge, F Besser, G Grossman, A Similar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis. |
| title | Similar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis. |
| title_full | Similar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis. |
| title_fullStr | Similar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis. |
| title_full_unstemmed | Similar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis. |
| title_short | Similar high molecular weight forms of growth hormone-releasing hormone are found in rat brain and testis. |
| title_sort | similar high molecular weight forms of growth hormone releasing hormone are found in rat brain and testis |
| work_keys_str_mv | AT tsagarakiss similarhighmolecularweightformsofgrowthhormonereleasinghormonearefoundinratbrainandtestis AT gef similarhighmolecularweightformsofgrowthhormonereleasinghormonearefoundinratbrainandtestis AT besserg similarhighmolecularweightformsofgrowthhormonereleasinghormonearefoundinratbrainandtestis AT grossmana similarhighmolecularweightformsofgrowthhormonereleasinghormonearefoundinratbrainandtestis |