Rhizopine biosensors for plant-dependent control of bacterial gene expression
Engineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (<...
| Κύριοι συγγραφείς: | , , , , , , , , , , , |
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| Μορφή: | Journal article |
| Γλώσσα: | English |
| Έκδοση: |
Wiley
2022
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| _version_ | 1826310162004049920 |
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| author | Haskett, TL Geddes, BA Paramasivan, P Green, P Chitnavis, S Mendes, MD Jorrín, B Knights, HE Bastholme, TR Ramsay, JP Oldroyd, GED Poole, PS |
| author_facet | Haskett, TL Geddes, BA Paramasivan, P Green, P Chitnavis, S Mendes, MD Jorrín, B Knights, HE Bastholme, TR Ramsay, JP Oldroyd, GED Poole, PS |
| author_sort | Haskett, TL |
| collection | OXFORD |
| description | Engineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (<i>RhiP</i>) plants and model bacterial strains. Here, we conduct an in-depth analysis of rhizopine-inducible expression in bacteria. We characterize two rhizopine-inducible promoters and explore the bacterial host-range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on <i>RhiP</i> barley roots compared to the previously described biosensor plasmid pSIR02. We demonstrate that a sub-population of <i>Azorhizobium caulinodans</i> cells carrying pSIR05 can sense rhizopine and activate gene expression when colonizing <i>RhiP</i> barley roots. However, these bacteria were mildly defective for colonization of <i>RhiP</i> barley roots compared to the wild-type parent strain. This work provides advancement towards establishing more robust plant-dependent control of bacterial gene expression and highlights the key challenges remaining to achieve this goal. |
| first_indexed | 2024-03-07T07:46:29Z |
| format | Journal article |
| id | oxford-uuid:22b48cf7-afa8-4af6-b30e-4cf4e6607af5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:46:29Z |
| publishDate | 2022 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:22b48cf7-afa8-4af6-b30e-4cf4e6607af52023-06-16T17:30:16ZRhizopine biosensors for plant-dependent control of bacterial gene expressionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:22b48cf7-afa8-4af6-b30e-4cf4e6607af5EnglishSymplectic ElementsWiley2022Haskett, TLGeddes, BAParamasivan, PGreen, PChitnavis, SMendes, MDJorrín, BKnights, HEBastholme, TRRamsay, JPOldroyd, GEDPoole, PSEngineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (<i>RhiP</i>) plants and model bacterial strains. Here, we conduct an in-depth analysis of rhizopine-inducible expression in bacteria. We characterize two rhizopine-inducible promoters and explore the bacterial host-range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on <i>RhiP</i> barley roots compared to the previously described biosensor plasmid pSIR02. We demonstrate that a sub-population of <i>Azorhizobium caulinodans</i> cells carrying pSIR05 can sense rhizopine and activate gene expression when colonizing <i>RhiP</i> barley roots. However, these bacteria were mildly defective for colonization of <i>RhiP</i> barley roots compared to the wild-type parent strain. This work provides advancement towards establishing more robust plant-dependent control of bacterial gene expression and highlights the key challenges remaining to achieve this goal. |
| spellingShingle | Haskett, TL Geddes, BA Paramasivan, P Green, P Chitnavis, S Mendes, MD Jorrín, B Knights, HE Bastholme, TR Ramsay, JP Oldroyd, GED Poole, PS Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_full | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_fullStr | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_full_unstemmed | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_short | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_sort | rhizopine biosensors for plant dependent control of bacterial gene expression |
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