Incidence of Prunus necrotic ring spot virus on Malus domestica in India
In surveys of apple (Malus domestica) orchards in various parts of Himachal Pradesh, samples from trees showing necrotic symptoms on the leaves were collected and tested for detection of Prunus necrotic ringspot virus (PNRSV) initially by ELISA followed by RT-PCR using coat protein gene primers. Pos...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1797058240137134080 |
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| author | Chandel, V Rana, T Handa, A Thakur, P Hallan, V Zaidi, A |
| author_facet | Chandel, V Rana, T Handa, A Thakur, P Hallan, V Zaidi, A |
| author_sort | Chandel, V |
| collection | OXFORD |
| description | In surveys of apple (Malus domestica) orchards in various parts of Himachal Pradesh, samples from trees showing necrotic symptoms on the leaves were collected and tested for detection of Prunus necrotic ringspot virus (PNRSV) initially by ELISA followed by RT-PCR using coat protein gene primers. Positive results were obtained in samples from Kullu and Kalpa regions. The virus gene sequences showed 88-97% similarity to corresponding sequences of other PNRSV isolates deposited in the GenBank database using ncbi.nih.nlm.gov. Although the similarity was high, there were some distinct differences with the Spanish isolate. This is the first report of PNRSV in apple from India. |
| first_indexed | 2024-03-06T19:47:43Z |
| format | Journal article |
| id | oxford-uuid:22e450cf-79ed-4ddf-ac9f-6a8d4cdf9237 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:47:43Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:22e450cf-79ed-4ddf-ac9f-6a8d4cdf92372022-03-26T11:41:12ZIncidence of Prunus necrotic ring spot virus on Malus domestica in IndiaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:22e450cf-79ed-4ddf-ac9f-6a8d4cdf9237EnglishSymplectic Elements at Oxford2008Chandel, VRana, THanda, AThakur, PHallan, VZaidi, AIn surveys of apple (Malus domestica) orchards in various parts of Himachal Pradesh, samples from trees showing necrotic symptoms on the leaves were collected and tested for detection of Prunus necrotic ringspot virus (PNRSV) initially by ELISA followed by RT-PCR using coat protein gene primers. Positive results were obtained in samples from Kullu and Kalpa regions. The virus gene sequences showed 88-97% similarity to corresponding sequences of other PNRSV isolates deposited in the GenBank database using ncbi.nih.nlm.gov. Although the similarity was high, there were some distinct differences with the Spanish isolate. This is the first report of PNRSV in apple from India. |
| spellingShingle | Chandel, V Rana, T Handa, A Thakur, P Hallan, V Zaidi, A Incidence of Prunus necrotic ring spot virus on Malus domestica in India |
| title | Incidence of Prunus necrotic ring spot virus on Malus domestica in India |
| title_full | Incidence of Prunus necrotic ring spot virus on Malus domestica in India |
| title_fullStr | Incidence of Prunus necrotic ring spot virus on Malus domestica in India |
| title_full_unstemmed | Incidence of Prunus necrotic ring spot virus on Malus domestica in India |
| title_short | Incidence of Prunus necrotic ring spot virus on Malus domestica in India |
| title_sort | incidence of prunus necrotic ring spot virus on malus domestica in india |
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