Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen.
To study the question when and where self peptides become associated with major histocompatibility complex class II molecules for tolerance induction, we recently developed a system in which the intracellular site(s) of antigen expression could be manipulated using gene cloning techniques. We previo...
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| Format: | Journal article |
| Language: | English |
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1991
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| _version_ | 1826263190374187008 |
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| author | Bikoff, E |
| author_facet | Bikoff, E |
| author_sort | Bikoff, E |
| collection | OXFORD |
| description | To study the question when and where self peptides become associated with major histocompatibility complex class II molecules for tolerance induction, we recently developed a system in which the intracellular site(s) of antigen expression could be manipulated using gene cloning techniques. We previously constructed a truncated IgGa gene comprising a variable (V) domain and the CH3 domain (not including the membrane exons) from the IgG2ab heavy (H) chain. The secreted form of the V-CH3b protein was expressed at high levels under control of the Ig H chain enhancer in Ia+ B lymphoma cells and was efficiently recognized by class II-restricted IgG2ab-specific T cell hybrids. Here we describe a modified V-CH3b gene construct in which the sequences encoding the signal peptide were deleted. A strong argument can be made that the signal-less V-CH3b protein is predominantly expressed in the cytosol. We show that transfected L cell lines expressing the signal-less form of the V-CH3b protein can stimulate class II-restricted IgG2ab-specific T cells. Cell mixing experiments indicate that this response cannot be due to passive uptake of soluble antigenic peptides released into culture supernatants. These experiments demonstrate that a cytoplasmic protein having no obvious means of reaching the cell surface can be presented to class II-restricted T cells. |
| first_indexed | 2024-03-06T19:47:47Z |
| format | Journal article |
| id | oxford-uuid:22ea96b3-8f7c-4f89-b70c-f239c16fe914 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:47:47Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:22ea96b3-8f7c-4f89-b70c-f239c16fe9142022-03-26T11:41:18ZClass II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:22ea96b3-8f7c-4f89-b70c-f239c16fe914EnglishSymplectic Elements at Oxford1991Bikoff, ETo study the question when and where self peptides become associated with major histocompatibility complex class II molecules for tolerance induction, we recently developed a system in which the intracellular site(s) of antigen expression could be manipulated using gene cloning techniques. We previously constructed a truncated IgGa gene comprising a variable (V) domain and the CH3 domain (not including the membrane exons) from the IgG2ab heavy (H) chain. The secreted form of the V-CH3b protein was expressed at high levels under control of the Ig H chain enhancer in Ia+ B lymphoma cells and was efficiently recognized by class II-restricted IgG2ab-specific T cell hybrids. Here we describe a modified V-CH3b gene construct in which the sequences encoding the signal peptide were deleted. A strong argument can be made that the signal-less V-CH3b protein is predominantly expressed in the cytosol. We show that transfected L cell lines expressing the signal-less form of the V-CH3b protein can stimulate class II-restricted IgG2ab-specific T cells. Cell mixing experiments indicate that this response cannot be due to passive uptake of soluble antigenic peptides released into culture supernatants. These experiments demonstrate that a cytoplasmic protein having no obvious means of reaching the cell surface can be presented to class II-restricted T cells. |
| spellingShingle | Bikoff, E Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen. |
| title | Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen. |
| title_full | Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen. |
| title_fullStr | Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen. |
| title_full_unstemmed | Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen. |
| title_short | Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen. |
| title_sort | class ii restricted igg2ab specific t cells recognize a signal minus form of the v ch3b antigen |
| work_keys_str_mv | AT bikoffe classiirestrictedigg2abspecifictcellsrecognizeasignalminusformofthevch3bantigen |