Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia.
We previously reported on the differential presence of transcripts related to the human endogenous retrovirus (HERV)-W family in cerebrospinal fluid and plasma from patients with first-episode schizophrenia compared with control individuals. Whether this is a consequence of qualitative or quantitati...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2008
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| _version_ | 1826263207361118208 |
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| author | Yao, Y Schröder, J Nellåker, C Bottmer, C Bachmann, S Yolken, R Karlsson, H |
| author_facet | Yao, Y Schröder, J Nellåker, C Bottmer, C Bachmann, S Yolken, R Karlsson, H |
| author_sort | Yao, Y |
| collection | OXFORD |
| description | We previously reported on the differential presence of transcripts related to the human endogenous retrovirus (HERV)-W family in cerebrospinal fluid and plasma from patients with first-episode schizophrenia compared with control individuals. Whether this is a consequence of qualitative or quantitative differences in transcription of genomic regions harboring HERV-W elements is not known. The purpose of the present study was therefore to characterize the transcribed HERV-W elements in mononuclear cells obtained from 30 patients first hospitalized for schizophrenia-related psychosis and from 26 healthy control individuals. We observed elevated total levels of HERV-W gag (2.1-fold, P < 0.01) but not env transcripts in the cells of patients compared with controls. By using the melting temperatures of the amplicons as a proxy marker for sequence identity, no absolute qualitative differences was detected between the two groups. Mapping of the detected transcripts identified several intronic and intergenic HERV-W elements transcribed in the cells, including elements previously considered transcriptionally silent. Element-specific assays revealed elevated levels of intronic transcripts containing HERV-W gag sequence from the putative gene PTD015 on chromosome 11q13.5 (1.6-fold, P < 0.05) in the patients compared with the controls. Thus, studies aiming to further understanding of complex human disease such as schizophrenia may need to be extended beyond the strictly protein-coding fraction of the transcriptome. |
| first_indexed | 2024-03-06T19:48:03Z |
| format | Journal article |
| id | oxford-uuid:23039b25-00c0-46e1-bde5-30d4e88b637c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:48:03Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:23039b25-00c0-46e1-bde5-30d4e88b637c2022-03-26T11:41:57ZElevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:23039b25-00c0-46e1-bde5-30d4e88b637cEnglishSymplectic Elements at Oxford2008Yao, YSchröder, JNellåker, CBottmer, CBachmann, SYolken, RKarlsson, HWe previously reported on the differential presence of transcripts related to the human endogenous retrovirus (HERV)-W family in cerebrospinal fluid and plasma from patients with first-episode schizophrenia compared with control individuals. Whether this is a consequence of qualitative or quantitative differences in transcription of genomic regions harboring HERV-W elements is not known. The purpose of the present study was therefore to characterize the transcribed HERV-W elements in mononuclear cells obtained from 30 patients first hospitalized for schizophrenia-related psychosis and from 26 healthy control individuals. We observed elevated total levels of HERV-W gag (2.1-fold, P < 0.01) but not env transcripts in the cells of patients compared with controls. By using the melting temperatures of the amplicons as a proxy marker for sequence identity, no absolute qualitative differences was detected between the two groups. Mapping of the detected transcripts identified several intronic and intergenic HERV-W elements transcribed in the cells, including elements previously considered transcriptionally silent. Element-specific assays revealed elevated levels of intronic transcripts containing HERV-W gag sequence from the putative gene PTD015 on chromosome 11q13.5 (1.6-fold, P < 0.05) in the patients compared with the controls. Thus, studies aiming to further understanding of complex human disease such as schizophrenia may need to be extended beyond the strictly protein-coding fraction of the transcriptome. |
| spellingShingle | Yao, Y Schröder, J Nellåker, C Bottmer, C Bachmann, S Yolken, R Karlsson, H Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia. |
| title | Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia. |
| title_full | Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia. |
| title_fullStr | Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia. |
| title_full_unstemmed | Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia. |
| title_short | Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia. |
| title_sort | elevated levels of human endogenous retrovirus w transcripts in blood cells from patients with first episode schizophrenia |
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