Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence.
The specificity (S) subunit of the restriction enzyme EcoKI imparts specificity for the sequence AAC(N6)GTGC. Substitution of thymine with bromodeoxyuridine in a 25 bp DNA duplex containing this sequence stimulated UV light-induced covalent crosslinking to the S subunit. Crosslinking occurred only a...
Main Authors: | Chen, A, Powell, L, Dryden, D, Murray, N, Brown, T |
---|---|
Format: | Journal article |
Language: | English |
Published: |
1995
|
Similar Items
-
IN VITRO BROMODEOXYURIDINE LABELING INDEX : PROGNOSTIC ROLE IN EARLY STAGE BREAST CANCER
by: Ersin Selçuk Ünal, et al.
Published: (2006-07-01) -
Cerebral cortex lesions decrease the number of bromodeoxyuridine-positive subventricular zone cells in mice.
by: Goings, G, et al.
Published: (2002) -
Bromodeoxyuridine Labelling to Determine Viral DNA Localization in Fluorescence and Electron Microscopy: The Case of Adenovirus
by: Gabriela N. Condezo, et al.
Published: (2021-09-01) -
Polypyrrole-Stabilized Polypeptide for Eco-Friendly Supercapacitors
by: Zhe Li, et al.
Published: (2023-01-01) -
Chitosan-polypeptide nanocomplexes crosslinked by a natural crosslinker enhanced the intestinal permeability of black tea polyphenol
by: Yike Jiang, et al.
Published: (2023-12-01)