Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans.
Fertilization in mammals requires sperm to bind to the zona pellucida (ZP) that surrounds the egg. Galactose (Gal) or N-acetylglucosamine (GlcNAc) residues on the glycans of ZP protein 3 (ZP3) have been implicated as mouse sperm receptors. However, Mgat1(-/-) eggs with modified N-glycans lacking ter...
| المؤلفون الرئيسيون: | , , , , |
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| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
2007
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| _version_ | 1826263262584373248 |
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| author | Williams, S Xia, L Cummings, R McEver, R Stanley, P |
| author_facet | Williams, S Xia, L Cummings, R McEver, R Stanley, P |
| author_sort | Williams, S |
| collection | OXFORD |
| description | Fertilization in mammals requires sperm to bind to the zona pellucida (ZP) that surrounds the egg. Galactose (Gal) or N-acetylglucosamine (GlcNAc) residues on the glycans of ZP protein 3 (ZP3) have been implicated as mouse sperm receptors. However, Mgat1(-/-) eggs with modified N-glycans lacking terminal Gal and GlcNAc residues are fertilized. To determine if Gal and GlcNAc on O-glycans of the ZP are required for fertilization, a conditional allele of the T-synthase gene (T-syn(F)) was generated. T-syn encodes core 1 beta1,3-galactosyltransferase 1 (T-synthase), which initiates the synthesis of core-1-derived O-glycans, the only O-glycans on mouse ZP3. T-syn(F/F):ZP3Cre females in which T-syn(F) was deleted at the beginning of oogenesis generated eggs lacking core-1-derived O-glycans. Nevertheless, T-syn(F/F):ZP3Cre females were fertile and their eggs bound sperm similarly to controls. In addition, T-syn(-/-) embryos generated from T-syn null eggs developed until approximately E12.5. Thus, core-1-derived O-glycans are not required for blastogenesis, implantation, or development prior to midgestation. Moreover, T-syn(-/-)Mgat1(-/-) eggs lacking complex and hybrid N-glycans as well as core-1-derived O-glycans were fertilized. The combined data show that mouse ZP3 does not require terminal Gal or GlcNAc on either N- or O-glycans for fertilization. |
| first_indexed | 2024-03-06T19:48:57Z |
| format | Journal article |
| id | oxford-uuid:234bc947-7147-45b9-bac1-15bcf33b8230 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:48:57Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:234bc947-7147-45b9-bac1-15bcf33b82302022-03-26T11:43:40ZFertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:234bc947-7147-45b9-bac1-15bcf33b8230EnglishSymplectic Elements at Oxford2007Williams, SXia, LCummings, RMcEver, RStanley, PFertilization in mammals requires sperm to bind to the zona pellucida (ZP) that surrounds the egg. Galactose (Gal) or N-acetylglucosamine (GlcNAc) residues on the glycans of ZP protein 3 (ZP3) have been implicated as mouse sperm receptors. However, Mgat1(-/-) eggs with modified N-glycans lacking terminal Gal and GlcNAc residues are fertilized. To determine if Gal and GlcNAc on O-glycans of the ZP are required for fertilization, a conditional allele of the T-synthase gene (T-syn(F)) was generated. T-syn encodes core 1 beta1,3-galactosyltransferase 1 (T-synthase), which initiates the synthesis of core-1-derived O-glycans, the only O-glycans on mouse ZP3. T-syn(F/F):ZP3Cre females in which T-syn(F) was deleted at the beginning of oogenesis generated eggs lacking core-1-derived O-glycans. Nevertheless, T-syn(F/F):ZP3Cre females were fertile and their eggs bound sperm similarly to controls. In addition, T-syn(-/-) embryos generated from T-syn null eggs developed until approximately E12.5. Thus, core-1-derived O-glycans are not required for blastogenesis, implantation, or development prior to midgestation. Moreover, T-syn(-/-)Mgat1(-/-) eggs lacking complex and hybrid N-glycans as well as core-1-derived O-glycans were fertilized. The combined data show that mouse ZP3 does not require terminal Gal or GlcNAc on either N- or O-glycans for fertilization. |
| spellingShingle | Williams, S Xia, L Cummings, R McEver, R Stanley, P Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans. |
| title | Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans. |
| title_full | Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans. |
| title_fullStr | Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans. |
| title_full_unstemmed | Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans. |
| title_short | Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans. |
| title_sort | fertilization in mouse does not require terminal galactose or n acetylglucosamine on the zona pellucida glycans |
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