HIV-1 recombination: an experimental assay and a phylogenetic approach.
The generation of genetic diversity is a fundamental characteristic of HIV-1 replication, allowing the virus to successfully evade the immune response and antiviral therapies. Although mutations are the first step towards diversity, mixing of the mutations through the process of recombination increa...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1797058427947581440 |
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| author | Moore, M Chin, M Hu, W |
| author_facet | Moore, M Chin, M Hu, W |
| author_sort | Moore, M |
| collection | OXFORD |
| description | The generation of genetic diversity is a fundamental characteristic of HIV-1 replication, allowing the virus to successfully evade the immune response and antiviral therapies. Although mutations are the first step towards diversity, mixing of the mutations through the process of recombination increases the variation and allows for the faster establishment of advantageous strains within the viral population. Therefore, studying recombination of HIV-1 provides insights into not only the mechanisms of HIV-1 replication but also into the potential for spread of antiviral drug resistance mutations within and across viral subtypes. This chapter describes, in detail, a highly sensitive recombination assay designed to measure the frequency of recombination between two viruses. This assay allows us to investigate the requirements, mechanisms, and final products of recombination. Additionally, software-based phylogenetic tools are described in this chapter, which allow for the identification of specific recombination events within patient samples or viral progeny from the recombination assay. |
| first_indexed | 2024-03-06T19:50:24Z |
| format | Journal article |
| id | oxford-uuid:23c85b2d-0baa-4ada-a0c0-0d4e4d315585 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:50:24Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:23c85b2d-0baa-4ada-a0c0-0d4e4d3155852022-03-26T11:46:04ZHIV-1 recombination: an experimental assay and a phylogenetic approach.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:23c85b2d-0baa-4ada-a0c0-0d4e4d315585EnglishSymplectic Elements at Oxford2009Moore, MChin, MHu, WThe generation of genetic diversity is a fundamental characteristic of HIV-1 replication, allowing the virus to successfully evade the immune response and antiviral therapies. Although mutations are the first step towards diversity, mixing of the mutations through the process of recombination increases the variation and allows for the faster establishment of advantageous strains within the viral population. Therefore, studying recombination of HIV-1 provides insights into not only the mechanisms of HIV-1 replication but also into the potential for spread of antiviral drug resistance mutations within and across viral subtypes. This chapter describes, in detail, a highly sensitive recombination assay designed to measure the frequency of recombination between two viruses. This assay allows us to investigate the requirements, mechanisms, and final products of recombination. Additionally, software-based phylogenetic tools are described in this chapter, which allow for the identification of specific recombination events within patient samples or viral progeny from the recombination assay. |
| spellingShingle | Moore, M Chin, M Hu, W HIV-1 recombination: an experimental assay and a phylogenetic approach. |
| title | HIV-1 recombination: an experimental assay and a phylogenetic approach. |
| title_full | HIV-1 recombination: an experimental assay and a phylogenetic approach. |
| title_fullStr | HIV-1 recombination: an experimental assay and a phylogenetic approach. |
| title_full_unstemmed | HIV-1 recombination: an experimental assay and a phylogenetic approach. |
| title_short | HIV-1 recombination: an experimental assay and a phylogenetic approach. |
| title_sort | hiv 1 recombination an experimental assay and a phylogenetic approach |
| work_keys_str_mv | AT moorem hiv1recombinationanexperimentalassayandaphylogeneticapproach AT chinm hiv1recombinationanexperimentalassayandaphylogeneticapproach AT huw hiv1recombinationanexperimentalassayandaphylogeneticapproach |