Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice
Interleukin (IL)-17 is emerging as an important cytokine in vaccine-induced protection against tuberculosis disease in animal models. Here we show that compared to parenteral delivery, BCG delivered mucosally enhances cytokine production, including interferon gamma and IL-17, in the lungs. Furthermo...
| Auteurs principaux: | , , , , , |
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| Format: | Journal article |
| Langue: | English |
| Publié: |
Public Library of Science
2013
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| _version_ | 1826263432832221184 |
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| author | Griffiths, K Stylianou, E Poyntz, H Betts, G Fletcher, H McShane, H |
| author_facet | Griffiths, K Stylianou, E Poyntz, H Betts, G Fletcher, H McShane, H |
| author_sort | Griffiths, K |
| collection | OXFORD |
| description | Interleukin (IL)-17 is emerging as an important cytokine in vaccine-induced protection against tuberculosis disease in animal models. Here we show that compared to parenteral delivery, BCG delivered mucosally enhances cytokine production, including interferon gamma and IL-17, in the lungs. Furthermore, we find that cholera toxin, delivered mucosally along with BCG, further enhances IL-17 production by CD4+ T cells over mucosal BCG alone both in the lungs and systemically. This boosting effect of CT is also observed using a vaccine regimen of BCG followed by the candidate vaccine MVA85A. Using a murine Mycobacterium tuberculosis (M.tb) aerosol challenge model, we demonstrate the ability of cholera toxin delivered at the time of a priming BCG vaccination to improve protection against tuberculosis disease in a manner at least partially dependent on the observed increase in IL-17. This observed increase in IL-17 in the lungs has no adverse effect on lung pathology following M.tb challenge, indicating that IL-17 can safely be boosted in murine lungs in a vaccine/M.tb challenge setting. |
| first_indexed | 2024-03-06T19:51:40Z |
| format | Journal article |
| id | oxford-uuid:242c46b2-a08d-4e76-95d6-aae8377e2d76 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:51:40Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:242c46b2-a08d-4e76-95d6-aae8377e2d762022-03-26T11:48:28ZCholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in miceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:242c46b2-a08d-4e76-95d6-aae8377e2d76EnglishSymplectic Elements at OxfordPublic Library of Science2013Griffiths, KStylianou, EPoyntz, HBetts, GFletcher, HMcShane, HInterleukin (IL)-17 is emerging as an important cytokine in vaccine-induced protection against tuberculosis disease in animal models. Here we show that compared to parenteral delivery, BCG delivered mucosally enhances cytokine production, including interferon gamma and IL-17, in the lungs. Furthermore, we find that cholera toxin, delivered mucosally along with BCG, further enhances IL-17 production by CD4+ T cells over mucosal BCG alone both in the lungs and systemically. This boosting effect of CT is also observed using a vaccine regimen of BCG followed by the candidate vaccine MVA85A. Using a murine Mycobacterium tuberculosis (M.tb) aerosol challenge model, we demonstrate the ability of cholera toxin delivered at the time of a priming BCG vaccination to improve protection against tuberculosis disease in a manner at least partially dependent on the observed increase in IL-17. This observed increase in IL-17 in the lungs has no adverse effect on lung pathology following M.tb challenge, indicating that IL-17 can safely be boosted in murine lungs in a vaccine/M.tb challenge setting. |
| spellingShingle | Griffiths, K Stylianou, E Poyntz, H Betts, G Fletcher, H McShane, H Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice |
| title | Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice |
| title_full | Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice |
| title_fullStr | Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice |
| title_full_unstemmed | Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice |
| title_short | Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice |
| title_sort | cholera toxin enhances vaccine induced protection against mycobacterium tuberculosis challenge in mice |
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