Identification of Single Molecules in Aqueous Solution by Time-Resolved Fluorescence Anisotropy

Using a confocal epi-illuminated microscope with a polarizing beam splitter and dual-channel detection of single-molecule fluorescence induced by pulsed laser excitation, a new application of the three-dimensional, real-time spectroscopic technique BIFL (burst integrated fluorescence lifetime) is in...

Full description

Bibliographic Details
Main Authors: Schaflfer, J, Volkmer, A, Eggeling, C, Subramaniam, V, Striker, G, Seidel, C
Format: Journal article
Language:English
Published: 1999