Identification of Single Molecules in Aqueous Solution by Time-Resolved Fluorescence Anisotropy

Using a confocal epi-illuminated microscope with a polarizing beam splitter and dual-channel detection of single-molecule fluorescence induced by pulsed laser excitation, a new application of the three-dimensional, real-time spectroscopic technique BIFL (burst integrated fluorescence lifetime) is in...

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Detalhes bibliográficos
Main Authors: Schaflfer, J, Volkmer, A, Eggeling, C, Subramaniam, V, Striker, G, Seidel, C
Formato: Journal article
Idioma:English
Publicado em: 1999