Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Springer Nature
2021
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| _version_ | 1797058618896416768 |
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| author | Mendonça, L Howe, A Gilchrist, JB Sheng, Y Sun, D Knight, ML Zanetti-Domingues, LC Bateman, B Krebs, A-S Chen, L Radecke, J Li, VD Ni, T Kounatidis, I Koronfel, MA Szynkiewicz, M Harkiolaki, M Martin-Fernandez, ML James, W Zhang, P |
| author_facet | Mendonça, L Howe, A Gilchrist, JB Sheng, Y Sun, D Knight, ML Zanetti-Domingues, LC Bateman, B Krebs, A-S Chen, L Radecke, J Li, VD Ni, T Kounatidis, I Koronfel, MA Szynkiewicz, M Harkiolaki, M Martin-Fernandez, ML James, W Zhang, P |
| author_sort | Mendonça, L |
| collection | OXFORD |
| description | Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events – e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. |
| first_indexed | 2024-03-06T19:52:52Z |
| format | Journal article |
| id | oxford-uuid:248ef07d-5b04-444b-b01b-6169c1a20780 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:52:52Z |
| publishDate | 2021 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:248ef07d-5b04-444b-b01b-6169c1a207802022-03-26T11:50:44ZCorrelative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egressJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:248ef07d-5b04-444b-b01b-6169c1a20780EnglishSymplectic ElementsSpringer Nature2021Mendonça, LHowe, AGilchrist, JBSheng, YSun, DKnight, MLZanetti-Domingues, LCBateman, BKrebs, A-SChen, LRadecke, JLi, VDNi, TKounatidis, IKoronfel, MASzynkiewicz, MHarkiolaki, MMartin-Fernandez, MLJames, WZhang, PSince the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events – e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. |
| spellingShingle | Mendonça, L Howe, A Gilchrist, JB Sheng, Y Sun, D Knight, ML Zanetti-Domingues, LC Bateman, B Krebs, A-S Chen, L Radecke, J Li, VD Ni, T Kounatidis, I Koronfel, MA Szynkiewicz, M Harkiolaki, M Martin-Fernandez, ML James, W Zhang, P Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |
| title | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |
| title_full | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |
| title_fullStr | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |
| title_full_unstemmed | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |
| title_short | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |
| title_sort | correlative multi scale cryo imaging unveils sars cov 2 assembly and egress |
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