Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3'-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplico...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser:	El-Sagheer, A, Brown, T
Format:	Journal article
Sprache:	English
Veröffentlicht:	2009

Ähnliche Einträge

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation
von: Chen, J, et al.
Veröffentlicht: (2018)

A triazole linkage that mimics the DNA phosphodiester group in living systems
von: El-Sagheer, A, et al.
Veröffentlicht: (2015)

Effect of Molecular Crowding on DNA Polymerase Reactions along Unnatural DNA Templates
von: Shuntaro Takahashi, et al.
Veröffentlicht: (2020-09-01)

Optimisation of an asymmetric polymerase chain reaction assay for the amplification of single-stranded DNA from Wuchereria bancrofti for electrochemical detection
von: Vasuki Venkatesan, et al.
Veröffentlicht: (2013-09-01)

Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
von: Andrew F. Gardner, et al.
Veröffentlicht: (2019-04-01)

Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing
von: Chen, X, et al.
Veröffentlicht: (2014)

Efficient RNA synthesis by in vitro transcription of a triazole-modified DNA template.
von: el-Sagheer, A, et al.
Veröffentlicht: (2011)

Simplified Method for Preservation and Polymerase Chain Reaction-amplification of Trypanosoma cruzi DNA in Human Blood
von: Patricia L Dorn, et al.
Veröffentlicht: (1997-03-01)

Nucleic acid amplification: Alternative methods of polymerase chain reaction
von: Md Fakruddin, et al.
Veröffentlicht: (2013-01-01)

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification
von: Konstantin B. Ignatov, et al.
Veröffentlicht: (2014-08-01)

DNA extraction and polymerase chain reaction
von: Nalini Gupta
Veröffentlicht: (2019-01-01)

Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis
von: Dwi Veni Endarwati, et al.
Veröffentlicht: (2024-06-01)

Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
von: AL Cândido, et al.
Veröffentlicht: (2000-04-01)

Diagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin
von: Juliana de A Matos, et al.
Veröffentlicht: (2006-08-01)

Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
von: Susana Pedraza-Díaz, et al.
Veröffentlicht: (2001-02-01)

Rapid and efficient DNA strand cross-linking by click chemistry.
von: Kocalka, P, et al.
Veröffentlicht: (2008)

Cyclisation reactions for the synthesis of natural and unnatural piperidines
von: Barat, Viktor
Veröffentlicht: (2017)

HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes.
von: Wordsworth, B, et al.
Veröffentlicht: (1990)

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
von: Taemaitree, L, et al.
Veröffentlicht: (2019)

Synthesis and Properties of Oligonucleotides Containing LNA-Sulfamate and Sulfamide Backbone Linkages
von: Zengin Kurt, B, et al.
Veröffentlicht: (2024)

Searching for the ideal triazole: investigating the 1,5-triazole as a charge neutral DNA backbone mimic
von: Baker, YR, et al.
Veröffentlicht: (2019)

Optimisation of reverse transcription-polymerase chain reaction for double stranded RNA extraction / Jamiila Ismail
von: Ismail, Jamiila
Veröffentlicht: (2007)

DNA polymerase delta-dependent repair of DNA single strand breaks containing 3'-end proximal lesions.
von: Parsons, J, et al.
Veröffentlicht: (2007)

Genomic DNA extraction and amplification of Leishmania donovani using polymerase chain reaction (PCR) from archived, Giemsa- stained slides
von: A. Amarasinghe, et al.
Veröffentlicht: (2021-04-01)

Detection of Mycobacterium leprae DNA from archaeological skeletal remains in Japan using whole genome amplification and polymerase chain reaction.
von: Koichi Suzuki, et al.
Veröffentlicht: (2010-01-01)

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
von: Lapatrada Taemaitree, et al.
Veröffentlicht: (2019-04-01)

“Unnatural, unnatural, unnatural, unnatural unnatural” . . . but real? The Toolbox Murders (Dennis Donnelly, 1978) and the Exploitation of True Story Adaptations
von: Wickham Clayton
Veröffentlicht: (2016-07-01)

Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase
von: G.J. Hafner, et al.
Veröffentlicht: (2001-04-01)

Structure and dynamics of triazole-linked DNA: biocompatibility explained.
von: Dallmann, A, et al.
Veröffentlicht: (2011)

The polymerase chain reaction /
von: Mullis, Kary B., et al.
Veröffentlicht: (1994)

Polymerase Chain Reaction - Single Strand Conformation Polymorphism (PCR-SSCP) analysis of goat as2-casein
von: A. Caroli, et al.
Veröffentlicht: (2010-04-01)

Use of the Restriction Enzyme AvaI and Exo− Bst Polymerase in Strand Displacement Amplification
von: Maria A. Milla, et al.
Veröffentlicht: (1998-03-01)

Sequencing of simple sequence repeat anchored polymerase chain reaction amplification products of Biomphalaria glabrata
von: Roberta L Caldeira, et al.
Veröffentlicht: (2002-10-01)

Assessment of Cost-Efficient Thermocycler Prototype for Polymerase Chain Reaction and Loop-Mediated Isothermal Amplification
von: Kenny Lischer, et al.
Veröffentlicht: (2021-12-01)

Improved diagnostic potential of polymerase chain reaction by amplification of multiple gene targets in osteoarticular tuberculosis
von: Gopinath Palanisamy, et al.
Veröffentlicht: (2017-01-01)

A microheater on polyimide substrate for hand-held realtime microfluidic polymerase chain reaction amplification
von: Dae-Sik Lee, et al.
Veröffentlicht: (2019-10-01)

Impact of Amplification Efficiency Approaches on Telomere Length Measurement via Quantitative-Polymerase Chain Reaction
von: Waylon J. Hastings, et al.
Veröffentlicht: (2021-09-01)

Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification.
von: Guillaume Roux, et al.
Veröffentlicht: (2019-01-01)

Sex determination of cattle meat by polymerase chain reaction amplification of the amelogenin (AMELX/AMELY) gene
von: P. Gokulakrishnan, et al.
Veröffentlicht: (2012-01-01)

Novel Strategies to Optimize the Amplification of Single-Stranded DNA
von: Atef Nehdi, et al.
Veröffentlicht: (2020-05-01)