Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.
DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3'-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplico...
Váldodahkkit: | El-Sagheer, A, Brown, T |
---|---|
Materiálatiipa: | Journal article |
Giella: | English |
Almmustuhtton: |
2009
|
Geahča maid
-
Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation
Dahkki: Chen, J, et al.
Almmustuhtton: (2018) -
A triazole linkage that mimics the DNA phosphodiester group in living systems
Dahkki: El-Sagheer, A, et al.
Almmustuhtton: (2015) -
Effect of Molecular Crowding on DNA Polymerase Reactions along Unnatural DNA Templates
Dahkki: Shuntaro Takahashi, et al.
Almmustuhtton: (2020-09-01) -
Optimisation of an asymmetric polymerase chain reaction assay for the amplification of single-stranded DNA from Wuchereria bancrofti for electrochemical detection
Dahkki: Vasuki Venkatesan, et al.
Almmustuhtton: (2013-09-01) -
Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
Dahkki: Andrew F. Gardner, et al.
Almmustuhtton: (2019-04-01)