Investigating cellular recognition using CRISPR/Cas9 genetic screening
Neighbouring cells can recognise and communicate with each other by direct binding between cell surface receptor and ligand pairs. Examples of cellular recognition events include pathogen entry into a host cell, sperm–egg fusion, and self/nonself discrimination by the immune system. Despite growing...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Cell Press
2020
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| _version_ | 1797058831587475456 |
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| author | Chong, Z-S Wright, GJ Sharma, S |
| author_facet | Chong, Z-S Wright, GJ Sharma, S |
| author_sort | Chong, Z-S |
| collection | OXFORD |
| description | Neighbouring cells can recognise and communicate with each other by direct binding between cell surface receptor and ligand pairs. Examples of cellular recognition events include pathogen entry into a host cell, sperm–egg fusion, and self/nonself discrimination by the immune system. Despite growing appreciation of cell surface recognition molecules as potential therapeutic targets, identifying key factors contributing to cellular recognition remains technically challenging to perform on a genome-wide scale. Recently, genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) knockout or activation (CRISPR-KO/CRISPRa) screens have been applied to identify the molecular determinants of cellular recognition. In this review, we discuss how CRISPR-KO/CRISPRa screening has contributed to our understanding of cellular recognition processes, and how it can be applied to investigate these important interactions in a range of biological contexts. |
| first_indexed | 2024-03-06T19:55:55Z |
| format | Journal article |
| id | oxford-uuid:2594f8d6-6ec0-4dd5-97ec-eaaf2b8a1ad2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:55:55Z |
| publishDate | 2020 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:2594f8d6-6ec0-4dd5-97ec-eaaf2b8a1ad22022-03-26T11:56:20ZInvestigating cellular recognition using CRISPR/Cas9 genetic screeningJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2594f8d6-6ec0-4dd5-97ec-eaaf2b8a1ad2EnglishSymplectic ElementsCell Press2020Chong, Z-SWright, GJSharma, SNeighbouring cells can recognise and communicate with each other by direct binding between cell surface receptor and ligand pairs. Examples of cellular recognition events include pathogen entry into a host cell, sperm–egg fusion, and self/nonself discrimination by the immune system. Despite growing appreciation of cell surface recognition molecules as potential therapeutic targets, identifying key factors contributing to cellular recognition remains technically challenging to perform on a genome-wide scale. Recently, genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) knockout or activation (CRISPR-KO/CRISPRa) screens have been applied to identify the molecular determinants of cellular recognition. In this review, we discuss how CRISPR-KO/CRISPRa screening has contributed to our understanding of cellular recognition processes, and how it can be applied to investigate these important interactions in a range of biological contexts. |
| spellingShingle | Chong, Z-S Wright, GJ Sharma, S Investigating cellular recognition using CRISPR/Cas9 genetic screening |
| title | Investigating cellular recognition using CRISPR/Cas9 genetic screening |
| title_full | Investigating cellular recognition using CRISPR/Cas9 genetic screening |
| title_fullStr | Investigating cellular recognition using CRISPR/Cas9 genetic screening |
| title_full_unstemmed | Investigating cellular recognition using CRISPR/Cas9 genetic screening |
| title_short | Investigating cellular recognition using CRISPR/Cas9 genetic screening |
| title_sort | investigating cellular recognition using crispr cas9 genetic screening |
| work_keys_str_mv | AT chongzs investigatingcellularrecognitionusingcrisprcas9geneticscreening AT wrightgj investigatingcellularrecognitionusingcrisprcas9geneticscreening AT sharmas investigatingcellularrecognitionusingcrisprcas9geneticscreening |