Changes in gene expression associated with FTO overexpression in mice
Single nucleotide polymorphisms in the first intron of the fat-mass-and-obesity-related gene FTO are associated with increased body weight and adiposity. Increased expression of FTO is likely underlying this obesity phenotype, as mice with two additional copies of Fto (FTO-4 mice) exhibit increased...
| প্রধান লেখক: | , , , , , , , , |
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| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
Public Library of Science
2014
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| _version_ | 1826263733686501376 |
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| author | Merkestein, M McTaggart, J Lee, S Kramer, H McMurray, F Lafond, M Boutens, L Cox, R Ashcroft, F |
| author_facet | Merkestein, M McTaggart, J Lee, S Kramer, H McMurray, F Lafond, M Boutens, L Cox, R Ashcroft, F |
| author_sort | Merkestein, M |
| collection | OXFORD |
| description | Single nucleotide polymorphisms in the first intron of the fat-mass-and-obesity-related gene FTO are associated with increased body weight and adiposity. Increased expression of FTO is likely underlying this obesity phenotype, as mice with two additional copies of Fto (FTO-4 mice) exhibit increased adiposity and are hyperphagic. FTO is a demethylase of single stranded DNA and RNA, and one of its targets is the m6A modification in RNA, which might play a role in the regulation of gene expression. In this study, we aimed to examine the changes in gene expression that occur in FTO-4 mice in order to gain more insight into the underlying mechanisms by which FTO influences body weight and adiposity. Our results indicate an upregulation of anabolic pathways and a downregulation of catabolic pathways in FTO-4 mice. Interestingly, although genes involved in methylation were differentially regulated in skeletal muscle of FTO-4 mice, no effect of FTO overexpression on m6A methylation of total mRNA was detected. |
| first_indexed | 2024-03-06T19:56:31Z |
| format | Journal article |
| id | oxford-uuid:25cb2ead-bb0f-4a60-83f9-4a57b2b1b621 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:56:31Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:25cb2ead-bb0f-4a60-83f9-4a57b2b1b6212022-03-26T11:57:34ZChanges in gene expression associated with FTO overexpression in miceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:25cb2ead-bb0f-4a60-83f9-4a57b2b1b621EnglishSymplectic Elements at OxfordPublic Library of Science2014Merkestein, MMcTaggart, JLee, SKramer, HMcMurray, FLafond, MBoutens, LCox, RAshcroft, FSingle nucleotide polymorphisms in the first intron of the fat-mass-and-obesity-related gene FTO are associated with increased body weight and adiposity. Increased expression of FTO is likely underlying this obesity phenotype, as mice with two additional copies of Fto (FTO-4 mice) exhibit increased adiposity and are hyperphagic. FTO is a demethylase of single stranded DNA and RNA, and one of its targets is the m6A modification in RNA, which might play a role in the regulation of gene expression. In this study, we aimed to examine the changes in gene expression that occur in FTO-4 mice in order to gain more insight into the underlying mechanisms by which FTO influences body weight and adiposity. Our results indicate an upregulation of anabolic pathways and a downregulation of catabolic pathways in FTO-4 mice. Interestingly, although genes involved in methylation were differentially regulated in skeletal muscle of FTO-4 mice, no effect of FTO overexpression on m6A methylation of total mRNA was detected. |
| spellingShingle | Merkestein, M McTaggart, J Lee, S Kramer, H McMurray, F Lafond, M Boutens, L Cox, R Ashcroft, F Changes in gene expression associated with FTO overexpression in mice |
| title | Changes in gene expression associated with FTO overexpression in mice |
| title_full | Changes in gene expression associated with FTO overexpression in mice |
| title_fullStr | Changes in gene expression associated with FTO overexpression in mice |
| title_full_unstemmed | Changes in gene expression associated with FTO overexpression in mice |
| title_short | Changes in gene expression associated with FTO overexpression in mice |
| title_sort | changes in gene expression associated with fto overexpression in mice |
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