Objective-type dark-field illumination for scattering from microbeads.
We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-...
| 主要な著者: | , , , , , |
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| フォーマット: | Journal article |
| 言語: | English |
| 出版事項: |
2001
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| _version_ | 1826263739759853568 |
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| author | Braslavsky, I Amit, R Jaffar Ali, B Gileadi, O Oppenheim, A Stavans, J |
| author_facet | Braslavsky, I Amit, R Jaffar Ali, B Gileadi, O Oppenheim, A Stavans, J |
| author_sort | Braslavsky, I |
| collection | OXFORD |
| description | We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-to-noise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method's use by following the Brownian motion of small beads attached by short DNA tethers to a substrate. |
| first_indexed | 2024-03-06T19:56:37Z |
| format | Journal article |
| id | oxford-uuid:25d3d9db-781f-49e3-8ece-137e10475f72 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:56:37Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:25d3d9db-781f-49e3-8ece-137e10475f722022-03-26T11:57:43ZObjective-type dark-field illumination for scattering from microbeads.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:25d3d9db-781f-49e3-8ece-137e10475f72EnglishSymplectic Elements at Oxford2001Braslavsky, IAmit, RJaffar Ali, BGileadi, OOppenheim, AStavans, JWe introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-to-noise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method's use by following the Brownian motion of small beads attached by short DNA tethers to a substrate. |
| spellingShingle | Braslavsky, I Amit, R Jaffar Ali, B Gileadi, O Oppenheim, A Stavans, J Objective-type dark-field illumination for scattering from microbeads. |
| title | Objective-type dark-field illumination for scattering from microbeads. |
| title_full | Objective-type dark-field illumination for scattering from microbeads. |
| title_fullStr | Objective-type dark-field illumination for scattering from microbeads. |
| title_full_unstemmed | Objective-type dark-field illumination for scattering from microbeads. |
| title_short | Objective-type dark-field illumination for scattering from microbeads. |
| title_sort | objective type dark field illumination for scattering from microbeads |
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