Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia.
Tumor hypoxia induces the up-regulation of a gene program associated with angiogenesis, glycolysis, adaptation to pH, and apoptosis via the hypoxia-inducible transcription factors (Hifs) 1 and 2. Disruption of this pathway has been proposed as a cancer therapy. Here, we use short interfering RNAs to...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2003
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| _version_ | 1826263743693062144 |
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| author | Sowter, H Raval, R Moore, J Ratcliffe, P Harris, A |
| author_facet | Sowter, H Raval, R Moore, J Ratcliffe, P Harris, A |
| author_sort | Sowter, H |
| collection | OXFORD |
| description | Tumor hypoxia induces the up-regulation of a gene program associated with angiogenesis, glycolysis, adaptation to pH, and apoptosis via the hypoxia-inducible transcription factors (Hifs) 1 and 2. Disruption of this pathway has been proposed as a cancer therapy. Here, we use short interfering RNAs to compare specific inactivation of Hif-1alpha or Hif-2alpha and show markedly different cell type-specific effects on gene expression and cell migration. Remarkably, among a panel of hypoxia-inducible genes, responses were critically dependent on Hif-1 alpha but not Hif-2 alpha in both endothelial and breast cancer cells but critically dependent on Hif-2 alpha in renal carcinoma cells. |
| first_indexed | 2024-03-06T19:56:41Z |
| format | Journal article |
| id | oxford-uuid:25d90596-0ae8-4e57-bdbe-92293f4287b1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:56:41Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:25d90596-0ae8-4e57-bdbe-92293f4287b12022-03-26T11:57:49ZPredominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:25d90596-0ae8-4e57-bdbe-92293f4287b1EnglishSymplectic Elements at Oxford2003Sowter, HRaval, RMoore, JRatcliffe, PHarris, ATumor hypoxia induces the up-regulation of a gene program associated with angiogenesis, glycolysis, adaptation to pH, and apoptosis via the hypoxia-inducible transcription factors (Hifs) 1 and 2. Disruption of this pathway has been proposed as a cancer therapy. Here, we use short interfering RNAs to compare specific inactivation of Hif-1alpha or Hif-2alpha and show markedly different cell type-specific effects on gene expression and cell migration. Remarkably, among a panel of hypoxia-inducible genes, responses were critically dependent on Hif-1 alpha but not Hif-2 alpha in both endothelial and breast cancer cells but critically dependent on Hif-2 alpha in renal carcinoma cells. |
| spellingShingle | Sowter, H Raval, R Moore, J Ratcliffe, P Harris, A Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia. |
| title | Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia. |
| title_full | Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia. |
| title_fullStr | Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia. |
| title_full_unstemmed | Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia. |
| title_short | Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia. |
| title_sort | predominant role of hypoxia inducible transcription factor hif 1alpha versus hif 2alpha in regulation of the transcriptional response to hypoxia |
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