The structural basis of ZMPSTE24-dependent laminopathies.
Mutations in the nuclear membrane zinc metalloprotease ZMPSTE24 lead to diseases of lamin processing (laminopathies), such as the premature aging disease progeria and metabolic disorders. ZMPSTE24 processes prelamin A, a component of the nuclear lamina intermediate filaments, by cleaving it at two s...
| Principais autores: | , , , , , , , , , , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2013
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| _version_ | 1826263761197989888 |
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| author | Quigley, A Dong, Y Pike, A Dong, L Shrestha, L Berridge, G Stansfeld, P Sansom, MS Edwards, A Bountra, C von Delft, F Bullock, A Burgess-Brown, N Carpenter, E |
| author_facet | Quigley, A Dong, Y Pike, A Dong, L Shrestha, L Berridge, G Stansfeld, P Sansom, MS Edwards, A Bountra, C von Delft, F Bullock, A Burgess-Brown, N Carpenter, E |
| author_sort | Quigley, A |
| collection | OXFORD |
| description | Mutations in the nuclear membrane zinc metalloprotease ZMPSTE24 lead to diseases of lamin processing (laminopathies), such as the premature aging disease progeria and metabolic disorders. ZMPSTE24 processes prelamin A, a component of the nuclear lamina intermediate filaments, by cleaving it at two sites. Failure of this processing results in accumulation of farnesylated, membrane-associated prelamin A. The 3.4 angstrom crystal structure of human ZMPSTE24 has a seven transmembrane α-helical barrel structure, surrounding a large, water-filled, intramembrane chamber, capped by a zinc metalloprotease domain with the catalytic site facing into the chamber. The 3.8 angstrom structure of a complex with a CSIM tetrapeptide showed that the mode of binding of the substrate resembles that of an insect metalloprotease inhibitor in thermolysin. Laminopathy-associated mutations predicted to reduce ZMPSTE24 activity map to the zinc metalloprotease peptide-binding site and to the bottom of the chamber. |
| first_indexed | 2024-03-06T19:56:59Z |
| format | Journal article |
| id | oxford-uuid:25f2b34f-e9c1-4136-89d5-2abfd8e59e9c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:56:59Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:25f2b34f-e9c1-4136-89d5-2abfd8e59e9c2022-03-26T11:58:18ZThe structural basis of ZMPSTE24-dependent laminopathies.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:25f2b34f-e9c1-4136-89d5-2abfd8e59e9cEnglishSymplectic Elements at Oxford2013Quigley, ADong, YPike, ADong, LShrestha, LBerridge, GStansfeld, PSansom, MSEdwards, ABountra, Cvon Delft, FBullock, ABurgess-Brown, NCarpenter, EMutations in the nuclear membrane zinc metalloprotease ZMPSTE24 lead to diseases of lamin processing (laminopathies), such as the premature aging disease progeria and metabolic disorders. ZMPSTE24 processes prelamin A, a component of the nuclear lamina intermediate filaments, by cleaving it at two sites. Failure of this processing results in accumulation of farnesylated, membrane-associated prelamin A. The 3.4 angstrom crystal structure of human ZMPSTE24 has a seven transmembrane α-helical barrel structure, surrounding a large, water-filled, intramembrane chamber, capped by a zinc metalloprotease domain with the catalytic site facing into the chamber. The 3.8 angstrom structure of a complex with a CSIM tetrapeptide showed that the mode of binding of the substrate resembles that of an insect metalloprotease inhibitor in thermolysin. Laminopathy-associated mutations predicted to reduce ZMPSTE24 activity map to the zinc metalloprotease peptide-binding site and to the bottom of the chamber. |
| spellingShingle | Quigley, A Dong, Y Pike, A Dong, L Shrestha, L Berridge, G Stansfeld, P Sansom, MS Edwards, A Bountra, C von Delft, F Bullock, A Burgess-Brown, N Carpenter, E The structural basis of ZMPSTE24-dependent laminopathies. |
| title | The structural basis of ZMPSTE24-dependent laminopathies. |
| title_full | The structural basis of ZMPSTE24-dependent laminopathies. |
| title_fullStr | The structural basis of ZMPSTE24-dependent laminopathies. |
| title_full_unstemmed | The structural basis of ZMPSTE24-dependent laminopathies. |
| title_short | The structural basis of ZMPSTE24-dependent laminopathies. |
| title_sort | structural basis of zmpste24 dependent laminopathies |
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