Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein-protein interactions using global analysis
Förster resonance energy transfer (FRET) detected via fluorescence lifetime imaging microscopy (FLIM) and global analysis provide a way in which protein-protein interactions may be spatially localized and quantified within biological cells. The FRET efficiency and proportion of interacting molecules...
Main Authors: | Barber, P, Ameer-Beg, S, Gilbey, J, Carlin, L, Keppler, M, Ng, T, Vojnovic, B |
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Format: | Journal article |
Language: | English |
Published: |
2009
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